Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice.

Ischemia-reperfusion injury (IRI) is inevitable during kidney transplantation leading to oxidative stress and inflammation. We previously reported that preoperative fasting in young-lean male mice protects against IRI. Since patients are generally of older age with morbidities possibly leading to a...

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Autores principales:	Franny Jongbloed, Ron W F de Bruin, Jeroen L A Pennings, César Payán-Gómez, Sandra van den Engel, Conny T van Oostrom, Alain de Bruin, Jan H J Hoeijmakers, Harry van Steeg, Jan N M Ĳzermans, Martijn E T Dollé
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/abca3416cb024ce49d1289932593833f
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spelling	oai:doaj.org-article:abca3416cb024ce49d1289932593833f2021-11-11T08:21:51ZPreoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice.1932-620310.1371/journal.pone.0100853https://doaj.org/article/abca3416cb024ce49d1289932593833f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24959849/?tool=EBIhttps://doaj.org/toc/1932-6203Ischemia-reperfusion injury (IRI) is inevitable during kidney transplantation leading to oxidative stress and inflammation. We previously reported that preoperative fasting in young-lean male mice protects against IRI. Since patients are generally of older age with morbidities possibly leading to a different response to fasting, we investigated the effects of preoperative fasting on renal IRI in aged-overweight male and female mice. Male and female F1-FVB/C57BL6-hybrid mice, average age 73 weeks weighing 47.2 grams, were randomized to preoperative ad libitum feeding or 3 days fasting, followed by renal IRI. Body weight, kidney function and survival of the animals were monitored until day 28 postoperatively. Kidney histopathology was scored for all animals and gene expression profiles after fasting were analyzed in kidneys of young and aged male mice. Preoperative fasting significantly improved survival after renal IRI in both sexes compared with normal fed mice. Fasted groups had a better kidney function shown by lower serum urea levels after renal IRI. Histopathology showed less acute tubular necrosis and more regeneration in kidneys from fasted mice. A mRNA analysis indicated the involvement of metabolic processes including fatty acid oxidation and retinol metabolism, and the NRF2-mediated stress response. Similar to young-lean, healthy male mice, preoperative fasting protects against renal IRI in aged-overweight mice of both genders. These findings suggest a general protective response of fasting against renal IRI regardless of age, gender, body weight and genetic background. Therefore, fasting could be a non-invasive intervention inducing increased oxidative stress resistance in older and overweight patients as well.Franny JongbloedRon W F de BruinJeroen L A PenningsCésar Payán-GómezSandra van den EngelConny T van OostromAlain de BruinJan H J HoeijmakersHarry van SteegJan N M ĲzermansMartijn E T DolléPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e100853 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Franny Jongbloed Ron W F de Bruin Jeroen L A Pennings César Payán-Gómez Sandra van den Engel Conny T van Oostrom Alain de Bruin Jan H J Hoeijmakers Harry van Steeg Jan N M Ĳzermans Martijn E T Dollé Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice.
description	Ischemia-reperfusion injury (IRI) is inevitable during kidney transplantation leading to oxidative stress and inflammation. We previously reported that preoperative fasting in young-lean male mice protects against IRI. Since patients are generally of older age with morbidities possibly leading to a different response to fasting, we investigated the effects of preoperative fasting on renal IRI in aged-overweight male and female mice. Male and female F1-FVB/C57BL6-hybrid mice, average age 73 weeks weighing 47.2 grams, were randomized to preoperative ad libitum feeding or 3 days fasting, followed by renal IRI. Body weight, kidney function and survival of the animals were monitored until day 28 postoperatively. Kidney histopathology was scored for all animals and gene expression profiles after fasting were analyzed in kidneys of young and aged male mice. Preoperative fasting significantly improved survival after renal IRI in both sexes compared with normal fed mice. Fasted groups had a better kidney function shown by lower serum urea levels after renal IRI. Histopathology showed less acute tubular necrosis and more regeneration in kidneys from fasted mice. A mRNA analysis indicated the involvement of metabolic processes including fatty acid oxidation and retinol metabolism, and the NRF2-mediated stress response. Similar to young-lean, healthy male mice, preoperative fasting protects against renal IRI in aged-overweight mice of both genders. These findings suggest a general protective response of fasting against renal IRI regardless of age, gender, body weight and genetic background. Therefore, fasting could be a non-invasive intervention inducing increased oxidative stress resistance in older and overweight patients as well.
format	article
author	Franny Jongbloed Ron W F de Bruin Jeroen L A Pennings César Payán-Gómez Sandra van den Engel Conny T van Oostrom Alain de Bruin Jan H J Hoeijmakers Harry van Steeg Jan N M Ĳzermans Martijn E T Dollé
author_facet	Franny Jongbloed Ron W F de Bruin Jeroen L A Pennings César Payán-Gómez Sandra van den Engel Conny T van Oostrom Alain de Bruin Jan H J Hoeijmakers Harry van Steeg Jan N M Ĳzermans Martijn E T Dollé
author_sort	Franny Jongbloed
title	Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice.
title_short	Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice.
title_full	Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice.
title_fullStr	Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice.
title_full_unstemmed	Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice.
title_sort	preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/abca3416cb024ce49d1289932593833f
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Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice.

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