One-step construction of circularized nanodiscs using SpyCatcher-SpyTag

Circularised nanodiscs (cNDs) are able to stabilise large lipid bilayer patches and are used for structural and functional studies. Current techniques to build cNDs have numerous steps and low yields; here the authors report a single step construction method using the SpyCatcher-SpyTag system.

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Auteurs principaux: Shanwen Zhang, Qian Ren, Scott J. Novick, Timothy S. Strutzenberg, Patrick R. Griffin, Huan Bao
Format: article
Langue:EN
Publié: Nature Portfolio 2021
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Accès en ligne:https://doaj.org/article/abcf045a3b4d48039b28f9381ec9e24a
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