One-step construction of circularized nanodiscs using SpyCatcher-SpyTag
Circularised nanodiscs (cNDs) are able to stabilise large lipid bilayer patches and are used for structural and functional studies. Current techniques to build cNDs have numerous steps and low yields; here the authors report a single step construction method using the SpyCatcher-SpyTag system.
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Nature Portfolio
2021
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oai:doaj.org-article:abcf045a3b4d48039b28f9381ec9e24a2021-12-02T18:33:56ZOne-step construction of circularized nanodiscs using SpyCatcher-SpyTag10.1038/s41467-021-25737-72041-1723https://doaj.org/article/abcf045a3b4d48039b28f9381ec9e24a2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25737-7https://doaj.org/toc/2041-1723Circularised nanodiscs (cNDs) are able to stabilise large lipid bilayer patches and are used for structural and functional studies. Current techniques to build cNDs have numerous steps and low yields; here the authors report a single step construction method using the SpyCatcher-SpyTag system.Shanwen ZhangQian RenScott J. NovickTimothy S. StrutzenbergPatrick R. GriffinHuan BaoNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-9 (2021) |
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Science Q Shanwen Zhang Qian Ren Scott J. Novick Timothy S. Strutzenberg Patrick R. Griffin Huan Bao One-step construction of circularized nanodiscs using SpyCatcher-SpyTag |
description |
Circularised nanodiscs (cNDs) are able to stabilise large lipid bilayer patches and are used for structural and functional studies. Current techniques to build cNDs have numerous steps and low yields; here the authors report a single step construction method using the SpyCatcher-SpyTag system. |
format |
article |
author |
Shanwen Zhang Qian Ren Scott J. Novick Timothy S. Strutzenberg Patrick R. Griffin Huan Bao |
author_facet |
Shanwen Zhang Qian Ren Scott J. Novick Timothy S. Strutzenberg Patrick R. Griffin Huan Bao |
author_sort |
Shanwen Zhang |
title |
One-step construction of circularized nanodiscs using SpyCatcher-SpyTag |
title_short |
One-step construction of circularized nanodiscs using SpyCatcher-SpyTag |
title_full |
One-step construction of circularized nanodiscs using SpyCatcher-SpyTag |
title_fullStr |
One-step construction of circularized nanodiscs using SpyCatcher-SpyTag |
title_full_unstemmed |
One-step construction of circularized nanodiscs using SpyCatcher-SpyTag |
title_sort |
one-step construction of circularized nanodiscs using spycatcher-spytag |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/abcf045a3b4d48039b28f9381ec9e24a |
work_keys_str_mv |
AT shanwenzhang onestepconstructionofcircularizednanodiscsusingspycatcherspytag AT qianren onestepconstructionofcircularizednanodiscsusingspycatcherspytag AT scottjnovick onestepconstructionofcircularizednanodiscsusingspycatcherspytag AT timothysstrutzenberg onestepconstructionofcircularizednanodiscsusingspycatcherspytag AT patrickrgriffin onestepconstructionofcircularizednanodiscsusingspycatcherspytag AT huanbao onestepconstructionofcircularizednanodiscsusingspycatcherspytag |
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1718377938678710272 |