One-step construction of circularized nanodiscs using SpyCatcher-SpyTag

Circularised nanodiscs (cNDs) are able to stabilise large lipid bilayer patches and are used for structural and functional studies. Current techniques to build cNDs have numerous steps and low yields; here the authors report a single step construction method using the SpyCatcher-SpyTag system.

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Bibliographic Details
Main Authors:	Shanwen Zhang, Qian Ren, Scott J. Novick, Timothy S. Strutzenberg, Patrick R. Griffin, Huan Bao
Format:	article
Language:	EN
Published:	Nature Portfolio 2021
Subjects:	Science Q
Online Access:	https://doaj.org/article/abcf045a3b4d48039b28f9381ec9e24a
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id	oai:doaj.org-article:abcf045a3b4d48039b28f9381ec9e24a
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spelling	oai:doaj.org-article:abcf045a3b4d48039b28f9381ec9e24a2021-12-02T18:33:56ZOne-step construction of circularized nanodiscs using SpyCatcher-SpyTag10.1038/s41467-021-25737-72041-1723https://doaj.org/article/abcf045a3b4d48039b28f9381ec9e24a2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25737-7https://doaj.org/toc/2041-1723Circularised nanodiscs (cNDs) are able to stabilise large lipid bilayer patches and are used for structural and functional studies. Current techniques to build cNDs have numerous steps and low yields; here the authors report a single step construction method using the SpyCatcher-SpyTag system.Shanwen ZhangQian RenScott J. NovickTimothy S. StrutzenbergPatrick R. GriffinHuan BaoNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Shanwen Zhang Qian Ren Scott J. Novick Timothy S. Strutzenberg Patrick R. Griffin Huan Bao One-step construction of circularized nanodiscs using SpyCatcher-SpyTag
description	Circularised nanodiscs (cNDs) are able to stabilise large lipid bilayer patches and are used for structural and functional studies. Current techniques to build cNDs have numerous steps and low yields; here the authors report a single step construction method using the SpyCatcher-SpyTag system.
format	article
author	Shanwen Zhang Qian Ren Scott J. Novick Timothy S. Strutzenberg Patrick R. Griffin Huan Bao
author_facet	Shanwen Zhang Qian Ren Scott J. Novick Timothy S. Strutzenberg Patrick R. Griffin Huan Bao
author_sort	Shanwen Zhang
title	One-step construction of circularized nanodiscs using SpyCatcher-SpyTag
title_short	One-step construction of circularized nanodiscs using SpyCatcher-SpyTag
title_full	One-step construction of circularized nanodiscs using SpyCatcher-SpyTag
title_fullStr	One-step construction of circularized nanodiscs using SpyCatcher-SpyTag
title_full_unstemmed	One-step construction of circularized nanodiscs using SpyCatcher-SpyTag
title_sort	one-step construction of circularized nanodiscs using spycatcher-spytag
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/abcf045a3b4d48039b28f9381ec9e24a
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One-step construction of circularized nanodiscs using SpyCatcher-SpyTag

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