Two new cellulolytic fungal species isolated from a 19th-century art collection

Abstract The archive of the Universidad de Costa Rica maintains a nineteenth-century French collection of drawings and lithographs in which the biodeterioration by fungi is rampant. Because of nutritional conditions in which these fungi grew, we suspected that they possessed an ability to degrade ce...

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Autores principales:	Carolina Coronado-Ruiz, Roberto Avendaño, Efraín Escudero-Leyva, Geraldine Conejo-Barboza, Priscila Chaverri, Max Chavarría
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ac3580ee986e463eb68574a4036928d5
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spelling	oai:doaj.org-article:ac3580ee986e463eb68574a4036928d52021-12-02T12:32:22ZTwo new cellulolytic fungal species isolated from a 19th-century art collection10.1038/s41598-018-24934-72045-2322https://doaj.org/article/ac3580ee986e463eb68574a4036928d52018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-24934-7https://doaj.org/toc/2045-2322Abstract The archive of the Universidad de Costa Rica maintains a nineteenth-century French collection of drawings and lithographs in which the biodeterioration by fungi is rampant. Because of nutritional conditions in which these fungi grew, we suspected that they possessed an ability to degrade cellulose. In this work our goal was to isolate and identify the fungal species responsible for the biodegradation of a nineteenth-century art collection and determine their cellulolytic activity. Fungi were isolated using potato-dextrose-agar (PDA) and water-agar with carboxymethyl cellulose (CMC). The identification of the fungi was assessed through DNA sequencing (nrDNA ITS and α-actin regions) complemented with morphological analyses. Assays for cellulolytic activity were conducted with Gram’s iodine as dye. Nineteen isolates were obtained, of which seventeen were identified through DNA sequencing to species level, belonging mainly to genera Arthrinium, Aspergillus, Chaetomium, Cladosporium, Colletotrichum, Penicillium and Trichoderma. For two samples that could not be identified through their ITS and α-actin sequences, a morphological analysis was conducted; they were identified as new species, named Periconia epilithographicola sp. nov. and Coniochaeta cipronana sp. nov. Qualitative tests showed that the fungal collection presents important cellulolytic activity.Carolina Coronado-RuizRoberto AvendañoEfraín Escudero-LeyvaGeraldine Conejo-BarbozaPriscila ChaverriMax ChavarríaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carolina Coronado-Ruiz Roberto Avendaño Efraín Escudero-Leyva Geraldine Conejo-Barboza Priscila Chaverri Max Chavarría Two new cellulolytic fungal species isolated from a 19th-century art collection
description	Abstract The archive of the Universidad de Costa Rica maintains a nineteenth-century French collection of drawings and lithographs in which the biodeterioration by fungi is rampant. Because of nutritional conditions in which these fungi grew, we suspected that they possessed an ability to degrade cellulose. In this work our goal was to isolate and identify the fungal species responsible for the biodegradation of a nineteenth-century art collection and determine their cellulolytic activity. Fungi were isolated using potato-dextrose-agar (PDA) and water-agar with carboxymethyl cellulose (CMC). The identification of the fungi was assessed through DNA sequencing (nrDNA ITS and α-actin regions) complemented with morphological analyses. Assays for cellulolytic activity were conducted with Gram’s iodine as dye. Nineteen isolates were obtained, of which seventeen were identified through DNA sequencing to species level, belonging mainly to genera Arthrinium, Aspergillus, Chaetomium, Cladosporium, Colletotrichum, Penicillium and Trichoderma. For two samples that could not be identified through their ITS and α-actin sequences, a morphological analysis was conducted; they were identified as new species, named Periconia epilithographicola sp. nov. and Coniochaeta cipronana sp. nov. Qualitative tests showed that the fungal collection presents important cellulolytic activity.
format	article
author	Carolina Coronado-Ruiz Roberto Avendaño Efraín Escudero-Leyva Geraldine Conejo-Barboza Priscila Chaverri Max Chavarría
author_facet	Carolina Coronado-Ruiz Roberto Avendaño Efraín Escudero-Leyva Geraldine Conejo-Barboza Priscila Chaverri Max Chavarría
author_sort	Carolina Coronado-Ruiz
title	Two new cellulolytic fungal species isolated from a 19th-century art collection
title_short	Two new cellulolytic fungal species isolated from a 19th-century art collection
title_full	Two new cellulolytic fungal species isolated from a 19th-century art collection
title_fullStr	Two new cellulolytic fungal species isolated from a 19th-century art collection
title_full_unstemmed	Two new cellulolytic fungal species isolated from a 19th-century art collection
title_sort	two new cellulolytic fungal species isolated from a 19th-century art collection
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/ac3580ee986e463eb68574a4036928d5
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Two new cellulolytic fungal species isolated from a 19th-century art collection

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