Novel cryo-EM structure of an ADP-bound GroEL–GroES complex

Abstract The GroEL–GroES chaperonin complex is a bacterial protein folding system, functioning in an ATP-dependent manner. Upon ATP binding and hydrolysis, it undergoes multiple stages linked to substrate protein binding, folding and release. Structural methods helped to reveal several conformationa...

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Autores principales:	Sofia S. Kudryavtseva, Evgeny B. Pichkur, Igor A. Yaroshevich, Aleksandra A. Mamchur, Irina S. Panina, Andrei V. Moiseenko, Olga S. Sokolova, Vladimir I. Muronetz, Tatiana B. Stanishneva-Konovalova
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/accfa7162fbd4527a18ab1b47e228906
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spelling	oai:doaj.org-article:accfa7162fbd4527a18ab1b47e2289062021-12-02T18:33:47ZNovel cryo-EM structure of an ADP-bound GroEL–GroES complex10.1038/s41598-021-97657-x2045-2322https://doaj.org/article/accfa7162fbd4527a18ab1b47e2289062021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97657-xhttps://doaj.org/toc/2045-2322Abstract The GroEL–GroES chaperonin complex is a bacterial protein folding system, functioning in an ATP-dependent manner. Upon ATP binding and hydrolysis, it undergoes multiple stages linked to substrate protein binding, folding and release. Structural methods helped to reveal several conformational states and provide more information about the chaperonin functional cycle. Here, using cryo-EM we resolved two nucleotide-bound structures of the bullet-shaped GroEL–GroES1 complex at 3.4 Å resolution. The main difference between them is the relative orientation of their apical domains. Both structures contain nucleotides in cis and trans GroEL rings; in contrast to previously reported bullet-shaped complexes where nucleotides were only present in the cis ring. Our results suggest that the bound nucleotides correspond to ADP, and that such a state appears at low ATP:ADP ratios.Sofia S. KudryavtsevaEvgeny B. PichkurIgor A. YaroshevichAleksandra A. MamchurIrina S. PaninaAndrei V. MoiseenkoOlga S. SokolovaVladimir I. MuronetzTatiana B. Stanishneva-KonovalovaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sofia S. Kudryavtseva Evgeny B. Pichkur Igor A. Yaroshevich Aleksandra A. Mamchur Irina S. Panina Andrei V. Moiseenko Olga S. Sokolova Vladimir I. Muronetz Tatiana B. Stanishneva-Konovalova Novel cryo-EM structure of an ADP-bound GroEL–GroES complex
description	Abstract The GroEL–GroES chaperonin complex is a bacterial protein folding system, functioning in an ATP-dependent manner. Upon ATP binding and hydrolysis, it undergoes multiple stages linked to substrate protein binding, folding and release. Structural methods helped to reveal several conformational states and provide more information about the chaperonin functional cycle. Here, using cryo-EM we resolved two nucleotide-bound structures of the bullet-shaped GroEL–GroES1 complex at 3.4 Å resolution. The main difference between them is the relative orientation of their apical domains. Both structures contain nucleotides in cis and trans GroEL rings; in contrast to previously reported bullet-shaped complexes where nucleotides were only present in the cis ring. Our results suggest that the bound nucleotides correspond to ADP, and that such a state appears at low ATP:ADP ratios.
format	article
author	Sofia S. Kudryavtseva Evgeny B. Pichkur Igor A. Yaroshevich Aleksandra A. Mamchur Irina S. Panina Andrei V. Moiseenko Olga S. Sokolova Vladimir I. Muronetz Tatiana B. Stanishneva-Konovalova
author_facet	Sofia S. Kudryavtseva Evgeny B. Pichkur Igor A. Yaroshevich Aleksandra A. Mamchur Irina S. Panina Andrei V. Moiseenko Olga S. Sokolova Vladimir I. Muronetz Tatiana B. Stanishneva-Konovalova
author_sort	Sofia S. Kudryavtseva
title	Novel cryo-EM structure of an ADP-bound GroEL–GroES complex
title_short	Novel cryo-EM structure of an ADP-bound GroEL–GroES complex
title_full	Novel cryo-EM structure of an ADP-bound GroEL–GroES complex
title_fullStr	Novel cryo-EM structure of an ADP-bound GroEL–GroES complex
title_full_unstemmed	Novel cryo-EM structure of an ADP-bound GroEL–GroES complex
title_sort	novel cryo-em structure of an adp-bound groel–groes complex
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/accfa7162fbd4527a18ab1b47e228906
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Novel cryo-EM structure of an ADP-bound GroEL–GroES complex

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