Phages versus Antibiotics To Treat Infected Diabetic Wounds in a Mouse Model: a Microbiological and Microbiotic Evaluation

ABSTRACT Diabetes is marked by a range of complications, including chronic infections that can lead to limb amputation. The treatment of infected wounds is disrupted by arteriopathies that reduce tissue perfusion as well as by the critical development of bacterial resistance. We evaluated the impact...

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Autores principales:	Jean-François Huon, Emmanuel Montassier, Anne-Gaëlle Leroy, Matthieu Grégoire, Marie-Anne Vibet, Jocelyne Caillon, David Boutoille, Dominique Navas
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	bacteriophages microbiota wound Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/acdd8b5571244b70babeded43468dfa6
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spelling	oai:doaj.org-article:acdd8b5571244b70babeded43468dfa62021-12-02T19:47:36ZPhages versus Antibiotics To Treat Infected Diabetic Wounds in a Mouse Model: a Microbiological and Microbiotic Evaluation10.1128/mSystems.00542-202379-5077https://doaj.org/article/acdd8b5571244b70babeded43468dfa62020-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00542-20https://doaj.org/toc/2379-5077ABSTRACT Diabetes is marked by a range of complications, including chronic infections that can lead to limb amputation. The treatment of infected wounds is disrupted by arteriopathies that reduce tissue perfusion as well as by the critical development of bacterial resistance. We evaluated the impact of a local application of bacteriophages compared to that of a per os administration of amoxicillin-clavulanic acid in a mouse model of Staphylococcus aureus wound infection. We found that phage treatment resulted in improved clinical healing and a reduction in local bacterial load at 7 and 14 days postinfection. Unlike antibiotics, phage therapy did not deplete the intestinal microbiota of treated animals. Amoxicillin resulted in a reduction of alpha and beta diversities of the murine microbiota and disturbed architecture even 7 days after the end of treatment, whereas phage treatment did not impinge on the microbiota. IMPORTANCE The management of diabetic foot infections is frequently a dead end for surgeons and infectious disease specialists. When the pathogen to be treated is not resistant to conventional antibiotics, the latter tend to unbalance the intestinal microbiota, which is linked to multiple pathologies. A local treatment with bacteriophages, in addition to being as much or even more effective than antibiotics from a clinical and microbiological point of view, makes it possible to respect the patient’s microbiota. These results suggest that the use of this therapeutic alternative is a major avenue and that the introduction of recommendations for their use is now necessary.Jean-François HuonEmmanuel MontassierAnne-Gaëlle LeroyMatthieu GrégoireMarie-Anne VibetJocelyne CaillonDavid BoutoilleDominique NavasAmerican Society for MicrobiologyarticlebacteriophagesmicrobiotawoundMicrobiologyQR1-502ENmSystems, Vol 5, Iss 6 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteriophages microbiota wound Microbiology QR1-502
spellingShingle	bacteriophages microbiota wound Microbiology QR1-502 Jean-François Huon Emmanuel Montassier Anne-Gaëlle Leroy Matthieu Grégoire Marie-Anne Vibet Jocelyne Caillon David Boutoille Dominique Navas Phages versus Antibiotics To Treat Infected Diabetic Wounds in a Mouse Model: a Microbiological and Microbiotic Evaluation
description	ABSTRACT Diabetes is marked by a range of complications, including chronic infections that can lead to limb amputation. The treatment of infected wounds is disrupted by arteriopathies that reduce tissue perfusion as well as by the critical development of bacterial resistance. We evaluated the impact of a local application of bacteriophages compared to that of a per os administration of amoxicillin-clavulanic acid in a mouse model of Staphylococcus aureus wound infection. We found that phage treatment resulted in improved clinical healing and a reduction in local bacterial load at 7 and 14 days postinfection. Unlike antibiotics, phage therapy did not deplete the intestinal microbiota of treated animals. Amoxicillin resulted in a reduction of alpha and beta diversities of the murine microbiota and disturbed architecture even 7 days after the end of treatment, whereas phage treatment did not impinge on the microbiota. IMPORTANCE The management of diabetic foot infections is frequently a dead end for surgeons and infectious disease specialists. When the pathogen to be treated is not resistant to conventional antibiotics, the latter tend to unbalance the intestinal microbiota, which is linked to multiple pathologies. A local treatment with bacteriophages, in addition to being as much or even more effective than antibiotics from a clinical and microbiological point of view, makes it possible to respect the patient’s microbiota. These results suggest that the use of this therapeutic alternative is a major avenue and that the introduction of recommendations for their use is now necessary.
format	article
author	Jean-François Huon Emmanuel Montassier Anne-Gaëlle Leroy Matthieu Grégoire Marie-Anne Vibet Jocelyne Caillon David Boutoille Dominique Navas
author_facet	Jean-François Huon Emmanuel Montassier Anne-Gaëlle Leroy Matthieu Grégoire Marie-Anne Vibet Jocelyne Caillon David Boutoille Dominique Navas
author_sort	Jean-François Huon
title	Phages versus Antibiotics To Treat Infected Diabetic Wounds in a Mouse Model: a Microbiological and Microbiotic Evaluation
title_short	Phages versus Antibiotics To Treat Infected Diabetic Wounds in a Mouse Model: a Microbiological and Microbiotic Evaluation
title_full	Phages versus Antibiotics To Treat Infected Diabetic Wounds in a Mouse Model: a Microbiological and Microbiotic Evaluation
title_fullStr	Phages versus Antibiotics To Treat Infected Diabetic Wounds in a Mouse Model: a Microbiological and Microbiotic Evaluation
title_full_unstemmed	Phages versus Antibiotics To Treat Infected Diabetic Wounds in a Mouse Model: a Microbiological and Microbiotic Evaluation
title_sort	phages versus antibiotics to treat infected diabetic wounds in a mouse model: a microbiological and microbiotic evaluation
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/acdd8b5571244b70babeded43468dfa6
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Phages versus Antibiotics To Treat Infected Diabetic Wounds in a Mouse Model: a Microbiological and Microbiotic Evaluation

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