Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens

CRISPR/Cas9-mediated forward genetic screens and gene-trap mutagenesis screens in haploid cells are both powerful techniques to examine gene function. Here, the authors show the two approaches have high concordance and identify an uncharacterized gene, TXNDC11, which is involved in endoplasmic retic...

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Autores principales:	Richard T. Timms, Sam A. Menzies, Iva A. Tchasovnikarova, Lea C. Christensen, James C. Williamson, Robin Antrobus, Gordon Dougan, Lars Ellgaard, Paul J. Lehner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/ace455e4f2ee40ba943423c1d8c92321
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spelling	oai:doaj.org-article:ace455e4f2ee40ba943423c1d8c923212021-12-02T17:32:52ZGenetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens10.1038/ncomms117862041-1723https://doaj.org/article/ace455e4f2ee40ba943423c1d8c923212016-06-01T00:00:00Zhttps://doi.org/10.1038/ncomms11786https://doaj.org/toc/2041-1723CRISPR/Cas9-mediated forward genetic screens and gene-trap mutagenesis screens in haploid cells are both powerful techniques to examine gene function. Here, the authors show the two approaches have high concordance and identify an uncharacterized gene, TXNDC11, which is involved in endoplasmic reticulum-associated degradation.Richard T. TimmsSam A. MenziesIva A. TchasovnikarovaLea C. ChristensenJames C. WilliamsonRobin AntrobusGordon DouganLars EllgaardPaul J. LehnerNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-10 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Richard T. Timms Sam A. Menzies Iva A. Tchasovnikarova Lea C. Christensen James C. Williamson Robin Antrobus Gordon Dougan Lars Ellgaard Paul J. Lehner Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens
description	CRISPR/Cas9-mediated forward genetic screens and gene-trap mutagenesis screens in haploid cells are both powerful techniques to examine gene function. Here, the authors show the two approaches have high concordance and identify an uncharacterized gene, TXNDC11, which is involved in endoplasmic reticulum-associated degradation.
format	article
author	Richard T. Timms Sam A. Menzies Iva A. Tchasovnikarova Lea C. Christensen James C. Williamson Robin Antrobus Gordon Dougan Lars Ellgaard Paul J. Lehner
author_facet	Richard T. Timms Sam A. Menzies Iva A. Tchasovnikarova Lea C. Christensen James C. Williamson Robin Antrobus Gordon Dougan Lars Ellgaard Paul J. Lehner
author_sort	Richard T. Timms
title	Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens
title_short	Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens
title_full	Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens
title_fullStr	Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens
title_full_unstemmed	Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens
title_sort	genetic dissection of mammalian erad through comparative haploid and crispr forward genetic screens
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/ace455e4f2ee40ba943423c1d8c92321
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Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens

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