Critical Relevance of Stochastic Effects on Low-Bacterial-Biomass 16S rRNA Gene Analysis

Critical Relevance of Stochastic Effects on Low-Bacterial-Biomass 16S rRNA Gene Analysis

ABSTRACT The bacterial microbiome of human body sites, previously considered sterile, remains highly controversial because it can be challenging to isolate signal from noise when low-biomass samples are being analyzed. We tested the hypothesis that stochastic sequencing noise, separable from reagent...

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Auteurs principaux: John R. Erb-Downward, Nicole R. Falkowski, Jennifer C. D’Souza, Lisa M. McCloskey, Roderick A. McDonald, Christopher A. Brown, Kerby Shedden, Robert P. Dickson, Christine M. Freeman, Kathleen A. Stringer, Betsy Foxman, Gary B. Huffnagle, Jeffrey L. Curtis, Sara D. Adar
Format: article
Langue:EN
Publié: American Society for Microbiology 2020
Sujets:
16S rRNA gene
contamination
exhaled breath condensate
low biomass
lung microbiome
sequencing noise
Microbiology
QR1-502
Accès en ligne:https://doaj.org/article/ad2262303bef4b7eacca97265e84955c
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https://doaj.org/article/ad2262303bef4b7eacca97265e84955c

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