FRUCTOSE SYRUP OBTENTION FROM ENZYMATIC HYDROLYSED OF YAM STARCH (<I>Dioscorea alata</I> and <I>Dioscorea rotundata</I>)

This investigation evaluated the yield of fructose syrup production from extracted starch of two yam species ( Dioscorea alata , var. Diamond and Dioscorea rotundata , var. Espino). The experiment was conducted using an at random experimental design of multiple categorical factor analysis with two...

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Autores principales:	Jairo G. SALCEDO M., Everaldo J. MONTES M., José E. ZAPATA M., Didier A. MÁRQUEZ H., Miguel DIAZ A.
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2010
Materias:	alpha-amylase hydrolysis substrate yam yield. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/ad6281198d2b4aa58454b719853408af
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spelling	oai:doaj.org-article:ad6281198d2b4aa58454b719853408af2021-11-19T04:14:19ZFRUCTOSE SYRUP OBTENTION FROM ENZYMATIC HYDROLYSED OF YAM STARCH (<I>Dioscorea alata</I> and <I>Dioscorea rotundata</I>)0121-40042145-2660https://doaj.org/article/ad6281198d2b4aa58454b719853408af2010-11-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/7427https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660This investigation evaluated the yield of fructose syrup production from extracted starch of two yam species ( Dioscorea alata , var. Diamond and Dioscorea rotundata , var. Espino). The experiment was conducted using an at random experimental design of multiple categorical factor analysis with two factors: a substrate concentration factor at five levels: 10, 20, 30, 40, 50% p/v and the yam species factor at two levels ( Dioscorea alata , var Diamond and Dioscorea rotundata , var Espino). To obtain fructose, initially glucose syrups were prepared by enzymatic hydrolysis of yam starch using the enzymes of Novozymes®,alpha-amylase Termamyl 120L (liquefaction stage) and AMG 300L (saccharification stage). At this stage, dextrose maximum equivalents (ED) obtained were 98.28 and 96.84 for a substrate concentration of 30%w/v. In a later stage isomerisation from glucose syrups to fructose syrups using the immobilized enzyme Sweetzyme of Novozymes® was carried out. The best results were achieved with substrate concentrations of 30% p/v for both yam species, which showed fructose concentrations of 220.57 and 210.36 g/L with conversions of 71.46% and 67.28% in Espino and Diamond yams respectively. No statistically significant differences were observed between the yam species ( D. alata var. Diamante and D. rotundata var. Espino) at a confidence level of 5%, regarding the production of fructose syrup.Jairo G. SALCEDO M.Everaldo J. MONTES M.José E. ZAPATA M.Didier A. MÁRQUEZ H.Miguel DIAZ A.Universidad de Antioquiaarticlealpha-amylasehydrolysissubstrateyamyield.Food processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 17, Iss 3 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	alpha-amylase hydrolysis substrate yam yield. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	alpha-amylase hydrolysis substrate yam yield. Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Jairo G. SALCEDO M. Everaldo J. MONTES M. José E. ZAPATA M. Didier A. MÁRQUEZ H. Miguel DIAZ A. FRUCTOSE SYRUP OBTENTION FROM ENZYMATIC HYDROLYSED OF YAM STARCH (<I>Dioscorea alata</I> and <I>Dioscorea rotundata</I>)
description	This investigation evaluated the yield of fructose syrup production from extracted starch of two yam species ( Dioscorea alata , var. Diamond and Dioscorea rotundata , var. Espino). The experiment was conducted using an at random experimental design of multiple categorical factor analysis with two factors: a substrate concentration factor at five levels: 10, 20, 30, 40, 50% p/v and the yam species factor at two levels ( Dioscorea alata , var Diamond and Dioscorea rotundata , var Espino). To obtain fructose, initially glucose syrups were prepared by enzymatic hydrolysis of yam starch using the enzymes of Novozymes®,alpha-amylase Termamyl 120L (liquefaction stage) and AMG 300L (saccharification stage). At this stage, dextrose maximum equivalents (ED) obtained were 98.28 and 96.84 for a substrate concentration of 30%w/v. In a later stage isomerisation from glucose syrups to fructose syrups using the immobilized enzyme Sweetzyme of Novozymes® was carried out. The best results were achieved with substrate concentrations of 30% p/v for both yam species, which showed fructose concentrations of 220.57 and 210.36 g/L with conversions of 71.46% and 67.28% in Espino and Diamond yams respectively. No statistically significant differences were observed between the yam species ( D. alata var. Diamante and D. rotundata var. Espino) at a confidence level of 5%, regarding the production of fructose syrup.
format	article
author	Jairo G. SALCEDO M. Everaldo J. MONTES M. José E. ZAPATA M. Didier A. MÁRQUEZ H. Miguel DIAZ A.
author_facet	Jairo G. SALCEDO M. Everaldo J. MONTES M. José E. ZAPATA M. Didier A. MÁRQUEZ H. Miguel DIAZ A.
author_sort	Jairo G. SALCEDO M.
title	FRUCTOSE SYRUP OBTENTION FROM ENZYMATIC HYDROLYSED OF YAM STARCH (<I>Dioscorea alata</I> and <I>Dioscorea rotundata</I>)
title_short	FRUCTOSE SYRUP OBTENTION FROM ENZYMATIC HYDROLYSED OF YAM STARCH (<I>Dioscorea alata</I> and <I>Dioscorea rotundata</I>)
title_full	FRUCTOSE SYRUP OBTENTION FROM ENZYMATIC HYDROLYSED OF YAM STARCH (<I>Dioscorea alata</I> and <I>Dioscorea rotundata</I>)
title_fullStr	FRUCTOSE SYRUP OBTENTION FROM ENZYMATIC HYDROLYSED OF YAM STARCH (<I>Dioscorea alata</I> and <I>Dioscorea rotundata</I>)
title_full_unstemmed	FRUCTOSE SYRUP OBTENTION FROM ENZYMATIC HYDROLYSED OF YAM STARCH (<I>Dioscorea alata</I> and <I>Dioscorea rotundata</I>)
title_sort	fructose syrup obtention from enzymatic hydrolysed of yam starch (<i>dioscorea alata</i> and <i>dioscorea rotundata</i>)
publisher	Universidad de Antioquia
publishDate	2010
url	https://doaj.org/article/ad6281198d2b4aa58454b719853408af
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FRUCTOSE SYRUP OBTENTION FROM ENZYMATIC HYDROLYSED OF YAM STARCH (<I>Dioscorea alata</I> and <I>Dioscorea rotundata</I>)

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