Isothiocyanates are detected in human synovial fluid following broccoli consumption and can affect the tissues of the knee joint

Abstract Osteoarthritis is a major cause of disability and there is no current pharmaceutical treatment which can prevent the disease or slow its progression. Dietary advice or supplementation is clearly an attractive option since it has low toxicity and ease of implementation on a population level....

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Autores principales:	Rose Davidson, Sarah Gardner, Orla Jupp, Angela Bullough, Sue Butters, Laura Watts, Simon Donell, Maria Traka, Shikha Saha, Richard Mithen, Mandy Peffers, Peter Clegg, Yongping Bao, Aedin Cassidy, Ian Clark
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/af28d705502641fe997009748fb236a4
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spelling	oai:doaj.org-article:af28d705502641fe997009748fb236a42021-12-02T15:05:40ZIsothiocyanates are detected in human synovial fluid following broccoli consumption and can affect the tissues of the knee joint10.1038/s41598-017-03629-52045-2322https://doaj.org/article/af28d705502641fe997009748fb236a42017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03629-5https://doaj.org/toc/2045-2322Abstract Osteoarthritis is a major cause of disability and there is no current pharmaceutical treatment which can prevent the disease or slow its progression. Dietary advice or supplementation is clearly an attractive option since it has low toxicity and ease of implementation on a population level. We have previously demonstrated that sulforaphane, a dietary isothiocyanate derived from its glucosinolate precursor which is found in broccoli, can prevent cartilage destruction in cells, in in vitro and in vivo models of osteoarthritis. As the next phase of this research, we enrolled 40 patients with knee osteoarthritis undergoing total knee replacement into a proof-of-principle trial. Patients were randomised to either a low or high glucosinolate diet for 14 days prior to surgery. We detected ITCs in the synovial fluid of the high glucosinolate group, but not the low glucosinolate group. This was mirrored by an increase in ITCs and specifically sulforaphane in the plasma. Proteomic analysis of synovial fluid showed significantly distinct profiles between groups with 125 differentially expressed proteins. The functional consequence of this diet will now be tested in a clinical trial.Rose DavidsonSarah GardnerOrla JuppAngela BulloughSue ButtersLaura WattsSimon DonellMaria TrakaShikha SahaRichard MithenMandy PeffersPeter CleggYongping BaoAedin CassidyIan ClarkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rose Davidson Sarah Gardner Orla Jupp Angela Bullough Sue Butters Laura Watts Simon Donell Maria Traka Shikha Saha Richard Mithen Mandy Peffers Peter Clegg Yongping Bao Aedin Cassidy Ian Clark Isothiocyanates are detected in human synovial fluid following broccoli consumption and can affect the tissues of the knee joint
description	Abstract Osteoarthritis is a major cause of disability and there is no current pharmaceutical treatment which can prevent the disease or slow its progression. Dietary advice or supplementation is clearly an attractive option since it has low toxicity and ease of implementation on a population level. We have previously demonstrated that sulforaphane, a dietary isothiocyanate derived from its glucosinolate precursor which is found in broccoli, can prevent cartilage destruction in cells, in in vitro and in vivo models of osteoarthritis. As the next phase of this research, we enrolled 40 patients with knee osteoarthritis undergoing total knee replacement into a proof-of-principle trial. Patients were randomised to either a low or high glucosinolate diet for 14 days prior to surgery. We detected ITCs in the synovial fluid of the high glucosinolate group, but not the low glucosinolate group. This was mirrored by an increase in ITCs and specifically sulforaphane in the plasma. Proteomic analysis of synovial fluid showed significantly distinct profiles between groups with 125 differentially expressed proteins. The functional consequence of this diet will now be tested in a clinical trial.
format	article
author	Rose Davidson Sarah Gardner Orla Jupp Angela Bullough Sue Butters Laura Watts Simon Donell Maria Traka Shikha Saha Richard Mithen Mandy Peffers Peter Clegg Yongping Bao Aedin Cassidy Ian Clark
author_facet	Rose Davidson Sarah Gardner Orla Jupp Angela Bullough Sue Butters Laura Watts Simon Donell Maria Traka Shikha Saha Richard Mithen Mandy Peffers Peter Clegg Yongping Bao Aedin Cassidy Ian Clark
author_sort	Rose Davidson
title	Isothiocyanates are detected in human synovial fluid following broccoli consumption and can affect the tissues of the knee joint
title_short	Isothiocyanates are detected in human synovial fluid following broccoli consumption and can affect the tissues of the knee joint
title_full	Isothiocyanates are detected in human synovial fluid following broccoli consumption and can affect the tissues of the knee joint
title_fullStr	Isothiocyanates are detected in human synovial fluid following broccoli consumption and can affect the tissues of the knee joint
title_full_unstemmed	Isothiocyanates are detected in human synovial fluid following broccoli consumption and can affect the tissues of the knee joint
title_sort	isothiocyanates are detected in human synovial fluid following broccoli consumption and can affect the tissues of the knee joint
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/af28d705502641fe997009748fb236a4
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Isothiocyanates are detected in human synovial fluid following broccoli consumption and can affect the tissues of the knee joint

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