Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins
Single fluorescent protein biosensors are susceptible to expression and instrumental artifacts. Here Ast et al. describe a dual fluorescent protein design whereby a reference fluorescent protein is nested within a reporter fluorescent protein to control for such artifacts while preserving sensitivit...
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Auteurs principaux: | , , , , , , |
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Format: | article |
Langue: | EN |
Publié: |
Nature Portfolio
2017
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Accès en ligne: | https://doaj.org/article/af5004125dba42869f7a394844689d77 |
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