Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins

Single fluorescent protein biosensors are susceptible to expression and instrumental artifacts. Here Ast et al. describe a dual fluorescent protein design whereby a reference fluorescent protein is nested within a reporter fluorescent protein to control for such artifacts while preserving sensitivit...

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Autores principales:	Cindy Ast, Jessica Foret, Luke M. Oltrogge, Roberto De Michele, Thomas J. Kleist, Cheng-Hsun Ho, Wolf B. Frommer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/af5004125dba42869f7a394844689d77
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spelling	oai:doaj.org-article:af5004125dba42869f7a394844689d772021-12-02T14:42:42ZRatiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins10.1038/s41467-017-00400-22041-1723https://doaj.org/article/af5004125dba42869f7a394844689d772017-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-00400-2https://doaj.org/toc/2041-1723Single fluorescent protein biosensors are susceptible to expression and instrumental artifacts. Here Ast et al. describe a dual fluorescent protein design whereby a reference fluorescent protein is nested within a reporter fluorescent protein to control for such artifacts while preserving sensitivity and dynamic range.Cindy AstJessica ForetLuke M. OltroggeRoberto De MicheleThomas J. KleistCheng-Hsun HoWolf B. FrommerNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Cindy Ast Jessica Foret Luke M. Oltrogge Roberto De Michele Thomas J. Kleist Cheng-Hsun Ho Wolf B. Frommer Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins
description	Single fluorescent protein biosensors are susceptible to expression and instrumental artifacts. Here Ast et al. describe a dual fluorescent protein design whereby a reference fluorescent protein is nested within a reporter fluorescent protein to control for such artifacts while preserving sensitivity and dynamic range.
format	article
author	Cindy Ast Jessica Foret Luke M. Oltrogge Roberto De Michele Thomas J. Kleist Cheng-Hsun Ho Wolf B. Frommer
author_facet	Cindy Ast Jessica Foret Luke M. Oltrogge Roberto De Michele Thomas J. Kleist Cheng-Hsun Ho Wolf B. Frommer
author_sort	Cindy Ast
title	Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins
title_short	Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins
title_full	Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins
title_fullStr	Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins
title_full_unstemmed	Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins
title_sort	ratiometric matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/af5004125dba42869f7a394844689d77
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Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins

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