Experimental strategies to achieve efficient targeted knock-in via tandem paired nicking

Experimental strategies to achieve efficient targeted knock-in via tandem paired nicking

Abstract Tandem paired nicking (TPN) is a method of genome editing that enables precise and relatively efficient targeted knock-in without appreciable restraint by p53-mediated DNA damage response. TPN is initiated by introducing two site-specific nicks on the same DNA strand using Cas9 nickases in...

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Bibliographic Details
Main Authors: Md. Lutfur Rahman, Toshinori Hyodo, Sivasundaram Karnan, Akinobu Ota, Muhammad Nazmul Hasan, Yuko Mihara, Md Wahiduzzaman, Shinobu Tsuzuki, Yoshitaka Hosokawa, Hiroyuki Konishi
Format: article
Language:EN
Published: Nature Portfolio 2021
Subjects:
Medicine
R
Science
Q
Online Access:https://doaj.org/article/b018edf2c7bd485b94b5fd71b1d3fa74
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