A CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station.

As we explore beyond Earth, astronauts may be at risk for harmful DNA damage caused by ionizing radiation. Double-strand breaks are a type of DNA damage that can be repaired by two major cellular pathways: non-homologous end joining, during which insertions or deletions may be added at the break sit...

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Autores principales:	Sarah Stahl-Rommel, David Li, Michelle Sung, Rebecca Li, Aarthi Vijayakumar, Kutay Deniz Atabay, G Guy Bushkin, Christian L Castro, Kevin D Foley, D Scott Copeland, Sarah L Castro-Wallace, Ezequiel Alvarez Saavedra, Emily J Gleason, Sebastian Kraves
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b05584a066a44f6ca8574d0d0e3317a4
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id	oai:doaj.org-article:b05584a066a44f6ca8574d0d0e3317a4
record_format	dspace
spelling	oai:doaj.org-article:b05584a066a44f6ca8574d0d0e3317a42021-12-02T20:15:45ZA CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station.1932-620310.1371/journal.pone.0253403https://doaj.org/article/b05584a066a44f6ca8574d0d0e3317a42021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253403https://doaj.org/toc/1932-6203As we explore beyond Earth, astronauts may be at risk for harmful DNA damage caused by ionizing radiation. Double-strand breaks are a type of DNA damage that can be repaired by two major cellular pathways: non-homologous end joining, during which insertions or deletions may be added at the break site, and homologous recombination, in which the DNA sequence often remains unchanged. Previous work suggests that space conditions may impact the choice of DNA repair pathway, potentially compounding the risks of increased radiation exposure during space travel. However, our understanding of this problem has been limited by technical and safety concerns, which have prevented integral study of the DNA repair process in space. The CRISPR/Cas9 gene editing system offers a model for the safe and targeted generation of double-strand breaks in eukaryotes. Here we describe a CRISPR-based assay for DNA break induction and assessment of double-strand break repair pathway choice entirely in space. As necessary steps in this process, we describe the first successful genetic transformation and CRISPR/Cas9 genome editing in space. These milestones represent a significant expansion of the molecular biology toolkit onboard the International Space Station.Sarah Stahl-RommelDavid LiMichelle SungRebecca LiAarthi VijayakumarKutay Deniz AtabayG Guy BushkinChristian L CastroKevin D FoleyD Scott CopelandSarah L Castro-WallaceEzequiel Alvarez SaavedraEmily J GleasonSebastian KravesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0253403 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sarah Stahl-Rommel David Li Michelle Sung Rebecca Li Aarthi Vijayakumar Kutay Deniz Atabay G Guy Bushkin Christian L Castro Kevin D Foley D Scott Copeland Sarah L Castro-Wallace Ezequiel Alvarez Saavedra Emily J Gleason Sebastian Kraves A CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station.
description	As we explore beyond Earth, astronauts may be at risk for harmful DNA damage caused by ionizing radiation. Double-strand breaks are a type of DNA damage that can be repaired by two major cellular pathways: non-homologous end joining, during which insertions or deletions may be added at the break site, and homologous recombination, in which the DNA sequence often remains unchanged. Previous work suggests that space conditions may impact the choice of DNA repair pathway, potentially compounding the risks of increased radiation exposure during space travel. However, our understanding of this problem has been limited by technical and safety concerns, which have prevented integral study of the DNA repair process in space. The CRISPR/Cas9 gene editing system offers a model for the safe and targeted generation of double-strand breaks in eukaryotes. Here we describe a CRISPR-based assay for DNA break induction and assessment of double-strand break repair pathway choice entirely in space. As necessary steps in this process, we describe the first successful genetic transformation and CRISPR/Cas9 genome editing in space. These milestones represent a significant expansion of the molecular biology toolkit onboard the International Space Station.
format	article
author	Sarah Stahl-Rommel David Li Michelle Sung Rebecca Li Aarthi Vijayakumar Kutay Deniz Atabay G Guy Bushkin Christian L Castro Kevin D Foley D Scott Copeland Sarah L Castro-Wallace Ezequiel Alvarez Saavedra Emily J Gleason Sebastian Kraves
author_facet	Sarah Stahl-Rommel David Li Michelle Sung Rebecca Li Aarthi Vijayakumar Kutay Deniz Atabay G Guy Bushkin Christian L Castro Kevin D Foley D Scott Copeland Sarah L Castro-Wallace Ezequiel Alvarez Saavedra Emily J Gleason Sebastian Kraves
author_sort	Sarah Stahl-Rommel
title	A CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station.
title_short	A CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station.
title_full	A CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station.
title_fullStr	A CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station.
title_full_unstemmed	A CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station.
title_sort	crispr-based assay for the study of eukaryotic dna repair onboard the international space station.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/b05584a066a44f6ca8574d0d0e3317a4
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A CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station.

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