Core Microbiome of Slovak Holstein Friesian Breeding Bulls’ Semen

Bacterial contamination of semen is an important factor connected to the health status of bulls that may significantly affect semen quality for artificial insemination. Moreover, some important bovine diseases may be transmitted through semen. Up to now, only a very limited number of complex studies...

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Autores principales:	Juraj Medo, Jana Žiarovská, Michal Ďuračka, Eva Tvrdá, Štefan Baňas, Michal Gábor, Matúš Kyseľ, Miroslava Kačániová
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	16S rRNA sequencing semen Holstein Friesian breeding bulls Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/b14401c2593b4cf8a7c09d5da018213a
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spelling	oai:doaj.org-article:b14401c2593b4cf8a7c09d5da018213a2021-11-25T16:21:39ZCore Microbiome of Slovak Holstein Friesian Breeding Bulls’ Semen10.3390/ani111133312076-2615https://doaj.org/article/b14401c2593b4cf8a7c09d5da018213a2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3331https://doaj.org/toc/2076-2615Bacterial contamination of semen is an important factor connected to the health status of bulls that may significantly affect semen quality for artificial insemination. Moreover, some important bovine diseases may be transmitted through semen. Up to now, only a very limited number of complex studies describing the semen microbiome of bulls have been published, as many bacteria are hard to cultivate using traditional techniques. The 16S rRNA high-throughput sequencing strategy allows for the reliable identification of bacterial profiles of bovine semen together with the detection of noncultivable bacterial species. Fresh samples from Holstein Friesian breeding bulls (<i>n</i> = 55) were examined for the natural variability in the present bacteria. Semen doses were selected randomly from Slovak Biological Services in Nitra, Slovak Republic. The most predominant phyla within the whole dataset were <i>Firmicutes</i> (31%), <i>Proteobacteria</i> (22%), <i>Fusobacteria</i> (18%), <i>Actinobacteria</i> (13%) and <i>Bacteroidetes</i> (12%). Samples of semen were divided into two separate clusters according to their microbiome compositions using a cording partition around a medoids analysis. Microbiomes of the first cluster (CL1) of samples (<i>n</i> = 20) were based on <i>Actinobacteria</i> (CL1 average = 25%; CL = 28%) and <i>Firmicutes</i> (CL1 = 38%; CL2 = 27%), while the second cluster (CL2; <i>n</i> = 35) contained samples characterized by a high prevalence of <i>Fusobacteria</i> (CL1 = 4%; CL2 = 26%). Some important indicator microbial groups were differentially distributed between the clusters.Juraj MedoJana ŽiarovskáMichal ĎuračkaEva TvrdáŠtefan BaňasMichal GáborMatúš KyseľMiroslava KačániováMDPI AGarticle16S rRNA sequencingsemenHolstein Friesian breeding bullsVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3331, p 3331 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	16S rRNA sequencing semen Holstein Friesian breeding bulls Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	16S rRNA sequencing semen Holstein Friesian breeding bulls Veterinary medicine SF600-1100 Zoology QL1-991 Juraj Medo Jana Žiarovská Michal Ďuračka Eva Tvrdá Štefan Baňas Michal Gábor Matúš Kyseľ Miroslava Kačániová Core Microbiome of Slovak Holstein Friesian Breeding Bulls’ Semen
description	Bacterial contamination of semen is an important factor connected to the health status of bulls that may significantly affect semen quality for artificial insemination. Moreover, some important bovine diseases may be transmitted through semen. Up to now, only a very limited number of complex studies describing the semen microbiome of bulls have been published, as many bacteria are hard to cultivate using traditional techniques. The 16S rRNA high-throughput sequencing strategy allows for the reliable identification of bacterial profiles of bovine semen together with the detection of noncultivable bacterial species. Fresh samples from Holstein Friesian breeding bulls (<i>n</i> = 55) were examined for the natural variability in the present bacteria. Semen doses were selected randomly from Slovak Biological Services in Nitra, Slovak Republic. The most predominant phyla within the whole dataset were <i>Firmicutes</i> (31%), <i>Proteobacteria</i> (22%), <i>Fusobacteria</i> (18%), <i>Actinobacteria</i> (13%) and <i>Bacteroidetes</i> (12%). Samples of semen were divided into two separate clusters according to their microbiome compositions using a cording partition around a medoids analysis. Microbiomes of the first cluster (CL1) of samples (<i>n</i> = 20) were based on <i>Actinobacteria</i> (CL1 average = 25%; CL = 28%) and <i>Firmicutes</i> (CL1 = 38%; CL2 = 27%), while the second cluster (CL2; <i>n</i> = 35) contained samples characterized by a high prevalence of <i>Fusobacteria</i> (CL1 = 4%; CL2 = 26%). Some important indicator microbial groups were differentially distributed between the clusters.
format	article
author	Juraj Medo Jana Žiarovská Michal Ďuračka Eva Tvrdá Štefan Baňas Michal Gábor Matúš Kyseľ Miroslava Kačániová
author_facet	Juraj Medo Jana Žiarovská Michal Ďuračka Eva Tvrdá Štefan Baňas Michal Gábor Matúš Kyseľ Miroslava Kačániová
author_sort	Juraj Medo
title	Core Microbiome of Slovak Holstein Friesian Breeding Bulls’ Semen
title_short	Core Microbiome of Slovak Holstein Friesian Breeding Bulls’ Semen
title_full	Core Microbiome of Slovak Holstein Friesian Breeding Bulls’ Semen
title_fullStr	Core Microbiome of Slovak Holstein Friesian Breeding Bulls’ Semen
title_full_unstemmed	Core Microbiome of Slovak Holstein Friesian Breeding Bulls’ Semen
title_sort	core microbiome of slovak holstein friesian breeding bulls’ semen
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b14401c2593b4cf8a7c09d5da018213a
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Core Microbiome of Slovak Holstein Friesian Breeding Bulls’ Semen

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