CELLULAR SUSPENSIONS ESTABLISHMENT OF <I>Bixa orellana</I> L., PHENOLIC COMPOUNDS PRODUCERS WITH POTENTIAL ANTIOPHIDIC ACTIVITY.

A protocol for production and massification of achiote cells in suspension from friable calli of leaf tissues, has been developed as a strategy to obtain antiophidics metabolites, especially phenolics compounds. Concentration effects of, inoculum, glucose, phosphorus and nitrogen, on cellular growth...

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Autores principales:	Silvia L. JIMÉNEZ R., Hader I. CASTAÑO P., Juan Carlos QUINTANA, Gelmy L. CIRO G., Lida QUINCHÍA, Sebastián ESTRADA
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2009
Materias:	cells suspensions Bixa orellana L phenolics compounds Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/b1acad2d79024de79193c14dcec0d38e
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spelling	oai:doaj.org-article:b1acad2d79024de79193c14dcec0d38e2021-11-19T04:18:49ZCELLULAR SUSPENSIONS ESTABLISHMENT OF <I>Bixa orellana</I> L., PHENOLIC COMPOUNDS PRODUCERS WITH POTENTIAL ANTIOPHIDIC ACTIVITY.0121-40042145-2660https://doaj.org/article/b1acad2d79024de79193c14dcec0d38e2009-02-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/768https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660A protocol for production and massification of achiote cells in suspension from friable calli of leaf tissues, has been developed as a strategy to obtain antiophidics metabolites, especially phenolics compounds. Concentration effects of, inoculum, glucose, phosphorus and nitrogen, on cellular growth kinetics were evaluated in ½ MS+2,4-D (5 ppm)+BAP (1 ppm) medium, stored at 25º C, darkness and 140 rpm, using a randomized factorial design with 4 factors, two levels and evaluation at 20 and 40 days of establishment. Treatment with highest biomass production of achiote cells in suspension, had an initial biomass concentration of 4g/l, glucose 20g/l, phosphorus 0,13g/l and nitrogen 2,52 g/l. Kinetics growth of achiote cells in suspension in culture conditions, presented a well defined exponential phase of 25 days, since then a stationary phase until the end of the evaluation (40 days). Total phenols contents among material obtained in-vitro and vegetable material obtained ex vitro were compared as a valid criteria to justify later works of in -vitro metabolic production in this vegetable specie. Silvia L. JIMÉNEZ R.Hader I. CASTAÑO P.Juan Carlos QUINTANAGelmy L. CIRO G.Lida QUINCHÍASebastián ESTRADAUniversidad de Antioquiaarticlecells suspensionsBixa orellana Lphenolics compoundsFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 15, Iss 1 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	cells suspensions Bixa orellana L phenolics compounds Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	cells suspensions Bixa orellana L phenolics compounds Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Silvia L. JIMÉNEZ R. Hader I. CASTAÑO P. Juan Carlos QUINTANA Gelmy L. CIRO G. Lida QUINCHÍA Sebastián ESTRADA CELLULAR SUSPENSIONS ESTABLISHMENT OF <I>Bixa orellana</I> L., PHENOLIC COMPOUNDS PRODUCERS WITH POTENTIAL ANTIOPHIDIC ACTIVITY.
description	A protocol for production and massification of achiote cells in suspension from friable calli of leaf tissues, has been developed as a strategy to obtain antiophidics metabolites, especially phenolics compounds. Concentration effects of, inoculum, glucose, phosphorus and nitrogen, on cellular growth kinetics were evaluated in ½ MS+2,4-D (5 ppm)+BAP (1 ppm) medium, stored at 25º C, darkness and 140 rpm, using a randomized factorial design with 4 factors, two levels and evaluation at 20 and 40 days of establishment. Treatment with highest biomass production of achiote cells in suspension, had an initial biomass concentration of 4g/l, glucose 20g/l, phosphorus 0,13g/l and nitrogen 2,52 g/l. Kinetics growth of achiote cells in suspension in culture conditions, presented a well defined exponential phase of 25 days, since then a stationary phase until the end of the evaluation (40 days). Total phenols contents among material obtained in-vitro and vegetable material obtained ex vitro were compared as a valid criteria to justify later works of in -vitro metabolic production in this vegetable specie.
format	article
author	Silvia L. JIMÉNEZ R. Hader I. CASTAÑO P. Juan Carlos QUINTANA Gelmy L. CIRO G. Lida QUINCHÍA Sebastián ESTRADA
author_facet	Silvia L. JIMÉNEZ R. Hader I. CASTAÑO P. Juan Carlos QUINTANA Gelmy L. CIRO G. Lida QUINCHÍA Sebastián ESTRADA
author_sort	Silvia L. JIMÉNEZ R.
title	CELLULAR SUSPENSIONS ESTABLISHMENT OF <I>Bixa orellana</I> L., PHENOLIC COMPOUNDS PRODUCERS WITH POTENTIAL ANTIOPHIDIC ACTIVITY.
title_short	CELLULAR SUSPENSIONS ESTABLISHMENT OF <I>Bixa orellana</I> L., PHENOLIC COMPOUNDS PRODUCERS WITH POTENTIAL ANTIOPHIDIC ACTIVITY.
title_full	CELLULAR SUSPENSIONS ESTABLISHMENT OF <I>Bixa orellana</I> L., PHENOLIC COMPOUNDS PRODUCERS WITH POTENTIAL ANTIOPHIDIC ACTIVITY.
title_fullStr	CELLULAR SUSPENSIONS ESTABLISHMENT OF <I>Bixa orellana</I> L., PHENOLIC COMPOUNDS PRODUCERS WITH POTENTIAL ANTIOPHIDIC ACTIVITY.
title_full_unstemmed	CELLULAR SUSPENSIONS ESTABLISHMENT OF <I>Bixa orellana</I> L., PHENOLIC COMPOUNDS PRODUCERS WITH POTENTIAL ANTIOPHIDIC ACTIVITY.
title_sort	cellular suspensions establishment of <i>bixa orellana</i> l., phenolic compounds producers with potential antiophidic activity.
publisher	Universidad de Antioquia
publishDate	2009
url	https://doaj.org/article/b1acad2d79024de79193c14dcec0d38e
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CELLULAR SUSPENSIONS ESTABLISHMENT OF <I>Bixa orellana</I> L., PHENOLIC COMPOUNDS PRODUCERS WITH POTENTIAL ANTIOPHIDIC ACTIVITY.

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