CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering

Abstract Genome engineering of bacteriophages provides opportunities for precise genetic dissection and for numerous phage applications including therapy. However, few methods are available for facile construction of unmarked precise deletions, insertions, gene replacements and point mutations in ba...

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Bibliographic Details
Main Authors: Katherine S. Wetzel, Carlos A. Guerrero-Bustamante, Rebekah M. Dedrick, Ching-Chung Ko, Krista G. Freeman, Haley G. Aull, Ashley M. Divens, Jeremy M. Rock, Kira M. Zack, Graham F. Hatfull
Format: article
Language:EN
Published: Nature Portfolio 2021
Subjects:
R
Q
Online Access:https://doaj.org/article/b1b89e6a2de841798e39f54d739bc4c6
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