CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering

Abstract Genome engineering of bacteriophages provides opportunities for precise genetic dissection and for numerous phage applications including therapy. However, few methods are available for facile construction of unmarked precise deletions, insertions, gene replacements and point mutations in ba...

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Autores principales:	Katherine S. Wetzel, Carlos A. Guerrero-Bustamante, Rebekah M. Dedrick, Ching-Chung Ko, Krista G. Freeman, Haley G. Aull, Ashley M. Divens, Jeremy M. Rock, Kira M. Zack, Graham F. Hatfull
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b1b89e6a2de841798e39f54d739bc4c6
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spelling	oai:doaj.org-article:b1b89e6a2de841798e39f54d739bc4c62021-12-02T17:04:08ZCRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering10.1038/s41598-021-86112-62045-2322https://doaj.org/article/b1b89e6a2de841798e39f54d739bc4c62021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86112-6https://doaj.org/toc/2045-2322Abstract Genome engineering of bacteriophages provides opportunities for precise genetic dissection and for numerous phage applications including therapy. However, few methods are available for facile construction of unmarked precise deletions, insertions, gene replacements and point mutations in bacteriophages for most bacterial hosts. Here we describe CRISPY-BRED and CRISPY-BRIP, methods for efficient and precise engineering of phages in Mycobacterium species, with applicability to phages of a variety of other hosts. This recombineering approach uses phage-derived recombination proteins and Streptococcus thermophilus CRISPR-Cas9.Katherine S. WetzelCarlos A. Guerrero-BustamanteRebekah M. DedrickChing-Chung KoKrista G. FreemanHaley G. AullAshley M. DivensJeremy M. RockKira M. ZackGraham F. HatfullNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katherine S. Wetzel Carlos A. Guerrero-Bustamante Rebekah M. Dedrick Ching-Chung Ko Krista G. Freeman Haley G. Aull Ashley M. Divens Jeremy M. Rock Kira M. Zack Graham F. Hatfull CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering
description	Abstract Genome engineering of bacteriophages provides opportunities for precise genetic dissection and for numerous phage applications including therapy. However, few methods are available for facile construction of unmarked precise deletions, insertions, gene replacements and point mutations in bacteriophages for most bacterial hosts. Here we describe CRISPY-BRED and CRISPY-BRIP, methods for efficient and precise engineering of phages in Mycobacterium species, with applicability to phages of a variety of other hosts. This recombineering approach uses phage-derived recombination proteins and Streptococcus thermophilus CRISPR-Cas9.
format	article
author	Katherine S. Wetzel Carlos A. Guerrero-Bustamante Rebekah M. Dedrick Ching-Chung Ko Krista G. Freeman Haley G. Aull Ashley M. Divens Jeremy M. Rock Kira M. Zack Graham F. Hatfull
author_facet	Katherine S. Wetzel Carlos A. Guerrero-Bustamante Rebekah M. Dedrick Ching-Chung Ko Krista G. Freeman Haley G. Aull Ashley M. Divens Jeremy M. Rock Kira M. Zack Graham F. Hatfull
author_sort	Katherine S. Wetzel
title	CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering
title_short	CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering
title_full	CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering
title_fullStr	CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering
title_full_unstemmed	CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering
title_sort	crispy-bred and crispy-brip: efficient bacteriophage engineering
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b1b89e6a2de841798e39f54d739bc4c6
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CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering

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