CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering

Abstract Genome engineering of bacteriophages provides opportunities for precise genetic dissection and for numerous phage applications including therapy. However, few methods are available for facile construction of unmarked precise deletions, insertions, gene replacements and point mutations in ba...

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Autores principales: Katherine S. Wetzel, Carlos A. Guerrero-Bustamante, Rebekah M. Dedrick, Ching-Chung Ko, Krista G. Freeman, Haley G. Aull, Ashley M. Divens, Jeremy M. Rock, Kira M. Zack, Graham F. Hatfull
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/b1b89e6a2de841798e39f54d739bc4c6
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