From cheese whey permeate to Sakacin-A/bacterial cellulose nanocrystal conjugates for antimicrobial food packaging applications: a circular economy case study

Abstract Applying a circular economy approach, this research explores the use of cheese whey permeate (CWP), by-product of whey ultrafiltration, as cheap substrate for the production of bacterial cellulose (BC) and Sakacin-A, to be used in an antimicrobial packaging material. BC from the acetic acid...

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Autores principales:	Manuela Rollini, Alida Musatti, Daniele Cavicchioli, Daniele Bussini, Stefano Farris, Cesare Rovera, Diego Romano, Stefano De Benedetti, Alberto Barbiroli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b257f5d0be234c0ab5a724f2e4f77d84
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spelling	oai:doaj.org-article:b257f5d0be234c0ab5a724f2e4f77d842021-12-02T11:41:41ZFrom cheese whey permeate to Sakacin-A/bacterial cellulose nanocrystal conjugates for antimicrobial food packaging applications: a circular economy case study10.1038/s41598-020-78430-y2045-2322https://doaj.org/article/b257f5d0be234c0ab5a724f2e4f77d842020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78430-yhttps://doaj.org/toc/2045-2322Abstract Applying a circular economy approach, this research explores the use of cheese whey permeate (CWP), by-product of whey ultrafiltration, as cheap substrate for the production of bacterial cellulose (BC) and Sakacin-A, to be used in an antimicrobial packaging material. BC from the acetic acid bacterium Komagataeibacter xylinus was boosted up to 6.77 g/L by supplementing CWP with β-galactosidase. BC was then reduced to nanocrystals (BCNCs, 70% conversion yield), which were then conjugated with Sakacin-A, an anti-Listeria bacteriocin produced by Lactobacillus sakei in a CWP based broth. Active conjugates (75 Activity Units (AU)/mg), an innovative solution for bacteriocin delivery, were then included in a coating mixture applied onto paper sheets at 25 AU/cm2. The obtained antimicrobial food package was found effective in reducing Listeria population in storage trials carried out on a fresh Italian soft cheese (named “stracchino”) intentionally inoculated with Listeria. Production costs of the active material have been mainly found to be associated (90%) to the purification steps. Setting a maximum prudential 50% cost reduction during process up-scaling, conjugates coating formulation would cost around 0.89 €/A4 sheet. Results represent a practical example of a circular economy production procedure by using a food industry by-product to produce antimicrobials for food preservation.Manuela RolliniAlida MusattiDaniele CavicchioliDaniele BussiniStefano FarrisCesare RoveraDiego RomanoStefano De BenedettiAlberto BarbiroliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manuela Rollini Alida Musatti Daniele Cavicchioli Daniele Bussini Stefano Farris Cesare Rovera Diego Romano Stefano De Benedetti Alberto Barbiroli From cheese whey permeate to Sakacin-A/bacterial cellulose nanocrystal conjugates for antimicrobial food packaging applications: a circular economy case study
description	Abstract Applying a circular economy approach, this research explores the use of cheese whey permeate (CWP), by-product of whey ultrafiltration, as cheap substrate for the production of bacterial cellulose (BC) and Sakacin-A, to be used in an antimicrobial packaging material. BC from the acetic acid bacterium Komagataeibacter xylinus was boosted up to 6.77 g/L by supplementing CWP with β-galactosidase. BC was then reduced to nanocrystals (BCNCs, 70% conversion yield), which were then conjugated with Sakacin-A, an anti-Listeria bacteriocin produced by Lactobacillus sakei in a CWP based broth. Active conjugates (75 Activity Units (AU)/mg), an innovative solution for bacteriocin delivery, were then included in a coating mixture applied onto paper sheets at 25 AU/cm2. The obtained antimicrobial food package was found effective in reducing Listeria population in storage trials carried out on a fresh Italian soft cheese (named “stracchino”) intentionally inoculated with Listeria. Production costs of the active material have been mainly found to be associated (90%) to the purification steps. Setting a maximum prudential 50% cost reduction during process up-scaling, conjugates coating formulation would cost around 0.89 €/A4 sheet. Results represent a practical example of a circular economy production procedure by using a food industry by-product to produce antimicrobials for food preservation.
format	article
author	Manuela Rollini Alida Musatti Daniele Cavicchioli Daniele Bussini Stefano Farris Cesare Rovera Diego Romano Stefano De Benedetti Alberto Barbiroli
author_facet	Manuela Rollini Alida Musatti Daniele Cavicchioli Daniele Bussini Stefano Farris Cesare Rovera Diego Romano Stefano De Benedetti Alberto Barbiroli
author_sort	Manuela Rollini
title	From cheese whey permeate to Sakacin-A/bacterial cellulose nanocrystal conjugates for antimicrobial food packaging applications: a circular economy case study
title_short	From cheese whey permeate to Sakacin-A/bacterial cellulose nanocrystal conjugates for antimicrobial food packaging applications: a circular economy case study
title_full	From cheese whey permeate to Sakacin-A/bacterial cellulose nanocrystal conjugates for antimicrobial food packaging applications: a circular economy case study
title_fullStr	From cheese whey permeate to Sakacin-A/bacterial cellulose nanocrystal conjugates for antimicrobial food packaging applications: a circular economy case study
title_full_unstemmed	From cheese whey permeate to Sakacin-A/bacterial cellulose nanocrystal conjugates for antimicrobial food packaging applications: a circular economy case study
title_sort	from cheese whey permeate to sakacin-a/bacterial cellulose nanocrystal conjugates for antimicrobial food packaging applications: a circular economy case study
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/b257f5d0be234c0ab5a724f2e4f77d84
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From cheese whey permeate to Sakacin-A/bacterial cellulose nanocrystal conjugates for antimicrobial food packaging applications: a circular economy case study

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