Mechanism and antibacterial activity of vine tea extract and dihydromyricetin against Staphylococcus aureus
Abstract Vine tea (Ampelopsis grossedentata) has been approved as a new food ingredient in 2013. Both vine tea extract (VTE) and its active ingredient, 2R, 3R-Dihydromyricetin (DMY), showed good antibacterial activity. The mechanism of VTE and DMY against Staphylococcus aureus were evaluated by morp...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2020
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/b2ae3525ba004fd6a9e21f6200d6ee64 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:b2ae3525ba004fd6a9e21f6200d6ee64 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:b2ae3525ba004fd6a9e21f6200d6ee642021-12-02T12:33:15ZMechanism and antibacterial activity of vine tea extract and dihydromyricetin against Staphylococcus aureus10.1038/s41598-020-78379-y2045-2322https://doaj.org/article/b2ae3525ba004fd6a9e21f6200d6ee642020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78379-yhttps://doaj.org/toc/2045-2322Abstract Vine tea (Ampelopsis grossedentata) has been approved as a new food ingredient in 2013. Both vine tea extract (VTE) and its active ingredient, 2R, 3R-Dihydromyricetin (DMY), showed good antibacterial activity. The mechanism of VTE and DMY against Staphylococcus aureus were evaluated by morphology observation, cell membrane and wall assay, protein assay, and DNA assay in this study. The results of SEM and TEM revealed that the VTE and DMY changed the morphology of S. aureus. The leakage of AKPase and β-galactosidase in treated groups demonstrated that the membrane integrity of S. aureus was disrupted. Meanwhile, the results of protein assay showed that VTE and DMY inhibited the expression of total proteins, and decreased activities of a few energy metabolism enzymes, total ATPase. Moreover, spectral and competitive analysis revealed that VTE and DMY interacted with DNA by groove and intercalation binding. Finally, the suspension experiments of Chinese cabbage and barley showed that inhibitors had strong inhibitory effect on bacteria growth. Overall, the results suggested that VTE and DMY may be potential food preservatives for inhibiting pathogen.Haiyun LiangKeke HeTing LiShumei CuiMeng TangShaoyi KangWei MaLiya SongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Haiyun Liang Keke He Ting Li Shumei Cui Meng Tang Shaoyi Kang Wei Ma Liya Song Mechanism and antibacterial activity of vine tea extract and dihydromyricetin against Staphylococcus aureus |
| description |
Abstract Vine tea (Ampelopsis grossedentata) has been approved as a new food ingredient in 2013. Both vine tea extract (VTE) and its active ingredient, 2R, 3R-Dihydromyricetin (DMY), showed good antibacterial activity. The mechanism of VTE and DMY against Staphylococcus aureus were evaluated by morphology observation, cell membrane and wall assay, protein assay, and DNA assay in this study. The results of SEM and TEM revealed that the VTE and DMY changed the morphology of S. aureus. The leakage of AKPase and β-galactosidase in treated groups demonstrated that the membrane integrity of S. aureus was disrupted. Meanwhile, the results of protein assay showed that VTE and DMY inhibited the expression of total proteins, and decreased activities of a few energy metabolism enzymes, total ATPase. Moreover, spectral and competitive analysis revealed that VTE and DMY interacted with DNA by groove and intercalation binding. Finally, the suspension experiments of Chinese cabbage and barley showed that inhibitors had strong inhibitory effect on bacteria growth. Overall, the results suggested that VTE and DMY may be potential food preservatives for inhibiting pathogen. |
| format |
article |
| author |
Haiyun Liang Keke He Ting Li Shumei Cui Meng Tang Shaoyi Kang Wei Ma Liya Song |
| author_facet |
Haiyun Liang Keke He Ting Li Shumei Cui Meng Tang Shaoyi Kang Wei Ma Liya Song |
| author_sort |
Haiyun Liang |
| title |
Mechanism and antibacterial activity of vine tea extract and dihydromyricetin against Staphylococcus aureus |
| title_short |
Mechanism and antibacterial activity of vine tea extract and dihydromyricetin against Staphylococcus aureus |
| title_full |
Mechanism and antibacterial activity of vine tea extract and dihydromyricetin against Staphylococcus aureus |
| title_fullStr |
Mechanism and antibacterial activity of vine tea extract and dihydromyricetin against Staphylococcus aureus |
| title_full_unstemmed |
Mechanism and antibacterial activity of vine tea extract and dihydromyricetin against Staphylococcus aureus |
| title_sort |
mechanism and antibacterial activity of vine tea extract and dihydromyricetin against staphylococcus aureus |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/b2ae3525ba004fd6a9e21f6200d6ee64 |
| work_keys_str_mv |
AT haiyunliang mechanismandantibacterialactivityofvineteaextractanddihydromyricetinagainststaphylococcusaureus AT kekehe mechanismandantibacterialactivityofvineteaextractanddihydromyricetinagainststaphylococcusaureus AT tingli mechanismandantibacterialactivityofvineteaextractanddihydromyricetinagainststaphylococcusaureus AT shumeicui mechanismandantibacterialactivityofvineteaextractanddihydromyricetinagainststaphylococcusaureus AT mengtang mechanismandantibacterialactivityofvineteaextractanddihydromyricetinagainststaphylococcusaureus AT shaoyikang mechanismandantibacterialactivityofvineteaextractanddihydromyricetinagainststaphylococcusaureus AT weima mechanismandantibacterialactivityofvineteaextractanddihydromyricetinagainststaphylococcusaureus AT liyasong mechanismandantibacterialactivityofvineteaextractanddihydromyricetinagainststaphylococcusaureus |
| _version_ |
1718393879824171008 |