Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes
CRISPR-Cas9 mediated gene editing has begun to revolutionize molecular biology, but editing efficiencies can vary greatly between reagents. The authors show that the addition of single-stranded non-homologous DNA stimulates gene disruption by favouring error-prone DNA repair.
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| Main Authors: | , , , |
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| Format: | article |
| Language: | EN |
| Published: |
Nature Portfolio
2016
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| Subjects: | |
| Online Access: | https://doaj.org/article/b52b9879e5a54a13a3e11a78f480fe3a |
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