Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes
CRISPR-Cas9 mediated gene editing has begun to revolutionize molecular biology, but editing efficiencies can vary greatly between reagents. The authors show that the addition of single-stranded non-homologous DNA stimulates gene disruption by favouring error-prone DNA repair.
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| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2016
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/b52b9879e5a54a13a3e11a78f480fe3a |
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| Sumario: | CRISPR-Cas9 mediated gene editing has begun to revolutionize molecular biology, but editing efficiencies can vary greatly between reagents. The authors show that the addition of single-stranded non-homologous DNA stimulates gene disruption by favouring error-prone DNA repair. |
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