Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes

CRISPR-Cas9 mediated gene editing has begun to revolutionize molecular biology, but editing efficiencies can vary greatly between reagents. The authors show that the addition of single-stranded non-homologous DNA stimulates gene disruption by favouring error-prone DNA repair.

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Bibliographic Details
Main Authors:	C. D. Richardson, G. J. Ray, N. L. Bray, J. E. Corn
Format:	article
Language:	EN
Published:	Nature Portfolio 2016
Subjects:	Science Q
Online Access:	https://doaj.org/article/b52b9879e5a54a13a3e11a78f480fe3a
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spelling	oai:doaj.org-article:b52b9879e5a54a13a3e11a78f480fe3a2021-12-02T14:38:36ZNon-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes10.1038/ncomms124632041-1723https://doaj.org/article/b52b9879e5a54a13a3e11a78f480fe3a2016-08-01T00:00:00Zhttps://doi.org/10.1038/ncomms12463https://doaj.org/toc/2041-1723CRISPR-Cas9 mediated gene editing has begun to revolutionize molecular biology, but editing efficiencies can vary greatly between reagents. The authors show that the addition of single-stranded non-homologous DNA stimulates gene disruption by favouring error-prone DNA repair.C. D. RichardsonG. J. RayN. L. BrayJ. E. CornNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-7 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q C. D. Richardson G. J. Ray N. L. Bray J. E. Corn Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes
description	CRISPR-Cas9 mediated gene editing has begun to revolutionize molecular biology, but editing efficiencies can vary greatly between reagents. The authors show that the addition of single-stranded non-homologous DNA stimulates gene disruption by favouring error-prone DNA repair.
format	article
author	C. D. Richardson G. J. Ray N. L. Bray J. E. Corn
author_facet	C. D. Richardson G. J. Ray N. L. Bray J. E. Corn
author_sort	C. D. Richardson
title	Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes
title_short	Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes
title_full	Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes
title_fullStr	Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes
title_full_unstemmed	Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes
title_sort	non-homologous dna increases gene disruption efficiency by altering dna repair outcomes
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/b52b9879e5a54a13a3e11a78f480fe3a
work_keys_str_mv	AT cdrichardson nonhomologousdnaincreasesgenedisruptionefficiencybyalteringdnarepairoutcomes AT gjray nonhomologousdnaincreasesgenedisruptionefficiencybyalteringdnarepairoutcomes AT nlbray nonhomologousdnaincreasesgenedisruptionefficiencybyalteringdnarepairoutcomes AT jecorn nonhomologousdnaincreasesgenedisruptionefficiencybyalteringdnarepairoutcomes
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Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes

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