Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes

CRISPR-Cas9 mediated gene editing has begun to revolutionize molecular biology, but editing efficiencies can vary greatly between reagents. The authors show that the addition of single-stranded non-homologous DNA stimulates gene disruption by favouring error-prone DNA repair.

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Autores principales: C. D. Richardson, G. J. Ray, N. L. Bray, J. E. Corn
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/b52b9879e5a54a13a3e11a78f480fe3a
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