Rational design of hyperstable antibacterial peptides for food preservation

Abstract We describe the design of peptides with properties like thermostability, pH stability, and antibacterial activity against a few bacterial food pathogens. Insights obtained from classical structure-function analysis of natural peptides and their mutants through antimicrobial and enzymatic as...

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Autores principales:	Yashavantha L. Vishweshwaraiah, Abhishek Acharya, Vinayak Hegde, Balaji Prakash
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
Acceso en línea:	https://doaj.org/article/b6792c9df54c45f4b71e34804df3d12e
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spelling	oai:doaj.org-article:b6792c9df54c45f4b71e34804df3d12e2021-12-02T16:38:51ZRational design of hyperstable antibacterial peptides for food preservation10.1038/s41538-021-00109-z2396-8370https://doaj.org/article/b6792c9df54c45f4b71e34804df3d12e2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41538-021-00109-zhttps://doaj.org/toc/2396-8370Abstract We describe the design of peptides with properties like thermostability, pH stability, and antibacterial activity against a few bacterial food pathogens. Insights obtained from classical structure-function analysis of natural peptides and their mutants through antimicrobial and enzymatic assays are used to rationally develop a set of peptides. pH and thermostability assays were performed to demonstrate robust antimicrobial activity post-treatment with high temperatures and at wide pH ranges. We have also investigated the mode of action of these hyperstable peptides using membrane permeability assays, electron microscopy, and molecular dynamics simulations. Notably, through mutational studies, we show that these peptides elicit their antibacterial action via both membrane destabilization and inhibition of intracellular trypsin—the two functions attributable to separate peptide segments. Finally, toxicity studies and food preservation assays demonstrate the safety and efficacy of the designed peptides for food preservation. Overall, the study provides a general ‘blueprint’ for the development of stable antimicrobial peptides (AMPs). Insights obtained from this work may also be combined with combinatorial methods in high-throughput studies for future development of antimicrobials for various applications.Yashavantha L. VishweshwaraiahAbhishek AcharyaVinayak HegdeBalaji PrakashNature PortfolioarticleNutrition. Foods and food supplyTX341-641Food processing and manufactureTP368-456ENnpj Science of Food, Vol 5, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
spellingShingle	Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456 Yashavantha L. Vishweshwaraiah Abhishek Acharya Vinayak Hegde Balaji Prakash Rational design of hyperstable antibacterial peptides for food preservation
description	Abstract We describe the design of peptides with properties like thermostability, pH stability, and antibacterial activity against a few bacterial food pathogens. Insights obtained from classical structure-function analysis of natural peptides and their mutants through antimicrobial and enzymatic assays are used to rationally develop a set of peptides. pH and thermostability assays were performed to demonstrate robust antimicrobial activity post-treatment with high temperatures and at wide pH ranges. We have also investigated the mode of action of these hyperstable peptides using membrane permeability assays, electron microscopy, and molecular dynamics simulations. Notably, through mutational studies, we show that these peptides elicit their antibacterial action via both membrane destabilization and inhibition of intracellular trypsin—the two functions attributable to separate peptide segments. Finally, toxicity studies and food preservation assays demonstrate the safety and efficacy of the designed peptides for food preservation. Overall, the study provides a general ‘blueprint’ for the development of stable antimicrobial peptides (AMPs). Insights obtained from this work may also be combined with combinatorial methods in high-throughput studies for future development of antimicrobials for various applications.
format	article
author	Yashavantha L. Vishweshwaraiah Abhishek Acharya Vinayak Hegde Balaji Prakash
author_facet	Yashavantha L. Vishweshwaraiah Abhishek Acharya Vinayak Hegde Balaji Prakash
author_sort	Yashavantha L. Vishweshwaraiah
title	Rational design of hyperstable antibacterial peptides for food preservation
title_short	Rational design of hyperstable antibacterial peptides for food preservation
title_full	Rational design of hyperstable antibacterial peptides for food preservation
title_fullStr	Rational design of hyperstable antibacterial peptides for food preservation
title_full_unstemmed	Rational design of hyperstable antibacterial peptides for food preservation
title_sort	rational design of hyperstable antibacterial peptides for food preservation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b6792c9df54c45f4b71e34804df3d12e
work_keys_str_mv	AT yashavanthalvishweshwaraiah rationaldesignofhyperstableantibacterialpeptidesforfoodpreservation AT abhishekacharya rationaldesignofhyperstableantibacterialpeptidesforfoodpreservation AT vinayakhegde rationaldesignofhyperstableantibacterialpeptidesforfoodpreservation AT balajiprakash rationaldesignofhyperstableantibacterialpeptidesforfoodpreservation
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Rational design of hyperstable antibacterial peptides for food preservation

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