Multiplex T-RFLP allows for increased target number and specificity: detection of Salmonella enterica and six species of Listeria in a single test.

A multiplex T-RFLP test was developed to detect and identify Salmonella enterica and all six species of Listeria inoculated into milk at minimal levels. Extensive in silico analysis was used to design a fifteen-primer, six-amplimer methodology and in vitro application showed target organism DNA, whe...

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Autores principales: Geoffrey N Elliott, Nadine Thomas, Marion Macrae, Colin D Campbell, Iain D Ogden, Brajesh K Singh
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2012
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Acceso en línea:https://doaj.org/article/b6be21108d864882abe6500d60a72db7
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