Understanding the Impact of Drought in <i>Coffea</i> Genotypes: Transcriptomic Analysis Supports a Common High Resilience to Moderate Water Deficit but a Genotype Dependent Sensitivity to Severe Water Deficit

Water scarcity is the most significant factor limiting coffee production, although some cultivars can still have important drought tolerance. This study analyzed leaf transcriptomes of two coffee cultivars with contrasting physiological responses, <i>Coffea canephora</i> cv. CL153 and &l...

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Autores principales:	Isabel Fernandes, Isabel Marques, Octávio S. Paulo, Dora Batista, Fábio L. Partelli, Fernando C. Lidon, Fábio M. DaMatta, José C. Ramalho, Ana I. Ribeiro-Barros
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ABA signaling climate changes coffee drought functional analysis leaf RNAseq Agriculture S
Acceso en línea:	https://doaj.org/article/b6e066563e964372bb28f9590b490717
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spelling	oai:doaj.org-article:b6e066563e964372bb28f9590b4907172021-11-25T16:09:00ZUnderstanding the Impact of Drought in <i>Coffea</i> Genotypes: Transcriptomic Analysis Supports a Common High Resilience to Moderate Water Deficit but a Genotype Dependent Sensitivity to Severe Water Deficit10.3390/agronomy111122552073-4395https://doaj.org/article/b6e066563e964372bb28f9590b4907172021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2255https://doaj.org/toc/2073-4395Water scarcity is the most significant factor limiting coffee production, although some cultivars can still have important drought tolerance. This study analyzed leaf transcriptomes of two coffee cultivars with contrasting physiological responses, <i>Coffea canephora</i> cv. CL153 and <i>Coffea. arabica</i> cv. Icatu, subjected to moderate (MWD) or severe water deficits (SWD). We found that MWD had a low impact compared with SWD, where 10% of all genes in Icatu and 17% in CL153 reacted to drought, being mainly down-regulated upon stress. Drought triggered a genotype-specific response involving the up-regulation of reticuline oxidase genes in CL153 and heat shock proteins in Icatu. Responsiveness to drought also included desiccation protectant genes, but primarily, aspartic proteases, especially in CL153. A total of 83 Transcription Factors were found engaged in response to drought, mainly up-regulated, especially under SWD. Together with the enrollment of 49 phosphatases and 272 protein kinases, results suggest the involvement of ABA-signaling processes in drought acclimation. The integration of these findings with complementing physiological and biochemical studies reveals that both genotypes are more resilient to moderate drought than previously thought and suggests the existence of post-transcriptional mechanisms modulating the response to drought.Isabel FernandesIsabel MarquesOctávio S. PauloDora BatistaFábio L. PartelliFernando C. LidonFábio M. DaMattaJosé C. RamalhoAna I. Ribeiro-BarrosMDPI AGarticleABA signalingclimate changescoffeedroughtfunctional analysisleaf RNAseqAgricultureSENAgronomy, Vol 11, Iss 2255, p 2255 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ABA signaling climate changes coffee drought functional analysis leaf RNAseq Agriculture S
spellingShingle	ABA signaling climate changes coffee drought functional analysis leaf RNAseq Agriculture S Isabel Fernandes Isabel Marques Octávio S. Paulo Dora Batista Fábio L. Partelli Fernando C. Lidon Fábio M. DaMatta José C. Ramalho Ana I. Ribeiro-Barros Understanding the Impact of Drought in <i>Coffea</i> Genotypes: Transcriptomic Analysis Supports a Common High Resilience to Moderate Water Deficit but a Genotype Dependent Sensitivity to Severe Water Deficit
description	Water scarcity is the most significant factor limiting coffee production, although some cultivars can still have important drought tolerance. This study analyzed leaf transcriptomes of two coffee cultivars with contrasting physiological responses, <i>Coffea canephora</i> cv. CL153 and <i>Coffea. arabica</i> cv. Icatu, subjected to moderate (MWD) or severe water deficits (SWD). We found that MWD had a low impact compared with SWD, where 10% of all genes in Icatu and 17% in CL153 reacted to drought, being mainly down-regulated upon stress. Drought triggered a genotype-specific response involving the up-regulation of reticuline oxidase genes in CL153 and heat shock proteins in Icatu. Responsiveness to drought also included desiccation protectant genes, but primarily, aspartic proteases, especially in CL153. A total of 83 Transcription Factors were found engaged in response to drought, mainly up-regulated, especially under SWD. Together with the enrollment of 49 phosphatases and 272 protein kinases, results suggest the involvement of ABA-signaling processes in drought acclimation. The integration of these findings with complementing physiological and biochemical studies reveals that both genotypes are more resilient to moderate drought than previously thought and suggests the existence of post-transcriptional mechanisms modulating the response to drought.
format	article
author	Isabel Fernandes Isabel Marques Octávio S. Paulo Dora Batista Fábio L. Partelli Fernando C. Lidon Fábio M. DaMatta José C. Ramalho Ana I. Ribeiro-Barros
author_facet	Isabel Fernandes Isabel Marques Octávio S. Paulo Dora Batista Fábio L. Partelli Fernando C. Lidon Fábio M. DaMatta José C. Ramalho Ana I. Ribeiro-Barros
author_sort	Isabel Fernandes
title	Understanding the Impact of Drought in <i>Coffea</i> Genotypes: Transcriptomic Analysis Supports a Common High Resilience to Moderate Water Deficit but a Genotype Dependent Sensitivity to Severe Water Deficit
title_short	Understanding the Impact of Drought in <i>Coffea</i> Genotypes: Transcriptomic Analysis Supports a Common High Resilience to Moderate Water Deficit but a Genotype Dependent Sensitivity to Severe Water Deficit
title_full	Understanding the Impact of Drought in <i>Coffea</i> Genotypes: Transcriptomic Analysis Supports a Common High Resilience to Moderate Water Deficit but a Genotype Dependent Sensitivity to Severe Water Deficit
title_fullStr	Understanding the Impact of Drought in <i>Coffea</i> Genotypes: Transcriptomic Analysis Supports a Common High Resilience to Moderate Water Deficit but a Genotype Dependent Sensitivity to Severe Water Deficit
title_full_unstemmed	Understanding the Impact of Drought in <i>Coffea</i> Genotypes: Transcriptomic Analysis Supports a Common High Resilience to Moderate Water Deficit but a Genotype Dependent Sensitivity to Severe Water Deficit
title_sort	understanding the impact of drought in <i>coffea</i> genotypes: transcriptomic analysis supports a common high resilience to moderate water deficit but a genotype dependent sensitivity to severe water deficit
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b6e066563e964372bb28f9590b490717
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Understanding the Impact of Drought in <i>Coffea</i> Genotypes: Transcriptomic Analysis Supports a Common High Resilience to Moderate Water Deficit but a Genotype Dependent Sensitivity to Severe Water Deficit

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