Metabolites of Prickly Rose: Chemodiversity and Digestive-Enzyme-Inhibiting Potential of Rosa acicularis and the Main Ellagitannin Rugosin D

Prickly rose (Rosaacicularis Lindl.) is the most distributed rose species in the Northern Hemisphere, used by indigenous people for various food purposes. The lack of detailed information about the chemical composition of R. acicularis has led...

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Autores principales:	Daniil N. Olennikov, Vladimir V. Chemposov, Nadezhda K. Chirikova
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Rosa acicularis</i> liquid chromatography–mass spectrometry metabolomics ellagitannins flavonoids rugosin D Botany QK1-989
Acceso en línea:	https://doaj.org/article/b7de8208c284412a884c0fa1618d259b
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spelling	oai:doaj.org-article:b7de8208c284412a884c0fa1618d259b2021-11-25T18:47:24ZMetabolites of Prickly Rose: Chemodiversity and Digestive-Enzyme-Inhibiting Potential of <i>Rosa acicularis</i> and the Main Ellagitannin Rugosin D10.3390/plants101125252223-7747https://doaj.org/article/b7de8208c284412a884c0fa1618d259b2021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2525https://doaj.org/toc/2223-7747Prickly rose (<i>Rosa</i><i>acicularis</i> Lindl.) is the most distributed rose species in the Northern Hemisphere, used by indigenous people for various food purposes. The lack of detailed information about the chemical composition of <i>R. acicularis</i> has led us to study the phytochemical composition and metabolic profile of prickly rose extracts using chromatographic techniques. Many groups of phenolic and non-phenolic compounds were quantified in the leaves, flowers, roots and fruits of <i>R. acicularis</i>. Phenolic compounds were the dominant phytochemicals in the aerial parts and roots of <i>R. acicularis</i>. A precise study by high-performance liquid chromatography with photodiode array detection and electrospray ionization triple quadrupole mass spectrometric detection showed the presence of 123 compounds, among which ellagic acid derivatives, ellagitannins, gallotannins, catechins, catechin oligomers, hydroxycinnamates and flavonoid glycosides of kaempferol, quercetin and dihydroquercetin were all identified for the first time. The most abundant phenolic compounds were ellagitannins and flavonoid glycosides, with a maximal content of 70.04 mg/g in leaves and 66.72 mg/g in flowers, respectively, indicating the great ability of <i>R. acicularis</i> organs to accumulate phenolic compounds. By applying a standardized static, simulated gastrointestinal digestion method, we found the inhibitory potential of the leaf extract against digestive α-amylases. A pancreatic α-amylase activity-inhibiting assay coupled with HPLC microfractionation demonstrated high inhibition of enzyme activity by ellagitannin rugosin D, which was later confirmed by a microplate reaction with mammalian α-amylases and the simulated digestion method. This study clearly demonstrates that <i>R. acicularis</i> leaf extract and its main component, ellagitannin rugosin D, strongly inhibit digestive α-amylase, and may be a prospective antidiabetic agent.Daniil N. OlennikovVladimir V. ChemposovNadezhda K. ChirikovaMDPI AGarticle<i>Rosa acicularis</i>liquid chromatography–mass spectrometrymetabolomicsellagitanninsflavonoidsrugosin DBotanyQK1-989ENPlants, Vol 10, Iss 2525, p 2525 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Rosa acicularis</i> liquid chromatography–mass spectrometry metabolomics ellagitannins flavonoids rugosin D Botany QK1-989
spellingShingle	<i>Rosa acicularis</i> liquid chromatography–mass spectrometry metabolomics ellagitannins flavonoids rugosin D Botany QK1-989 Daniil N. Olennikov Vladimir V. Chemposov Nadezhda K. Chirikova Metabolites of Prickly Rose: Chemodiversity and Digestive-Enzyme-Inhibiting Potential of <i>Rosa acicularis</i> and the Main Ellagitannin Rugosin D
description	Prickly rose (<i>Rosa</i><i>acicularis</i> Lindl.) is the most distributed rose species in the Northern Hemisphere, used by indigenous people for various food purposes. The lack of detailed information about the chemical composition of <i>R. acicularis</i> has led us to study the phytochemical composition and metabolic profile of prickly rose extracts using chromatographic techniques. Many groups of phenolic and non-phenolic compounds were quantified in the leaves, flowers, roots and fruits of <i>R. acicularis</i>. Phenolic compounds were the dominant phytochemicals in the aerial parts and roots of <i>R. acicularis</i>. A precise study by high-performance liquid chromatography with photodiode array detection and electrospray ionization triple quadrupole mass spectrometric detection showed the presence of 123 compounds, among which ellagic acid derivatives, ellagitannins, gallotannins, catechins, catechin oligomers, hydroxycinnamates and flavonoid glycosides of kaempferol, quercetin and dihydroquercetin were all identified for the first time. The most abundant phenolic compounds were ellagitannins and flavonoid glycosides, with a maximal content of 70.04 mg/g in leaves and 66.72 mg/g in flowers, respectively, indicating the great ability of <i>R. acicularis</i> organs to accumulate phenolic compounds. By applying a standardized static, simulated gastrointestinal digestion method, we found the inhibitory potential of the leaf extract against digestive α-amylases. A pancreatic α-amylase activity-inhibiting assay coupled with HPLC microfractionation demonstrated high inhibition of enzyme activity by ellagitannin rugosin D, which was later confirmed by a microplate reaction with mammalian α-amylases and the simulated digestion method. This study clearly demonstrates that <i>R. acicularis</i> leaf extract and its main component, ellagitannin rugosin D, strongly inhibit digestive α-amylase, and may be a prospective antidiabetic agent.
format	article
author	Daniil N. Olennikov Vladimir V. Chemposov Nadezhda K. Chirikova
author_facet	Daniil N. Olennikov Vladimir V. Chemposov Nadezhda K. Chirikova
author_sort	Daniil N. Olennikov
title	Metabolites of Prickly Rose: Chemodiversity and Digestive-Enzyme-Inhibiting Potential of <i>Rosa acicularis</i> and the Main Ellagitannin Rugosin D
title_short	Metabolites of Prickly Rose: Chemodiversity and Digestive-Enzyme-Inhibiting Potential of <i>Rosa acicularis</i> and the Main Ellagitannin Rugosin D
title_full	Metabolites of Prickly Rose: Chemodiversity and Digestive-Enzyme-Inhibiting Potential of <i>Rosa acicularis</i> and the Main Ellagitannin Rugosin D
title_fullStr	Metabolites of Prickly Rose: Chemodiversity and Digestive-Enzyme-Inhibiting Potential of <i>Rosa acicularis</i> and the Main Ellagitannin Rugosin D
title_full_unstemmed	Metabolites of Prickly Rose: Chemodiversity and Digestive-Enzyme-Inhibiting Potential of <i>Rosa acicularis</i> and the Main Ellagitannin Rugosin D
title_sort	metabolites of prickly rose: chemodiversity and digestive-enzyme-inhibiting potential of <i>rosa acicularis</i> and the main ellagitannin rugosin d
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b7de8208c284412a884c0fa1618d259b
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Metabolites of Prickly Rose: Chemodiversity and Digestive-Enzyme-Inhibiting Potential of <i>Rosa acicularis</i> and the Main Ellagitannin Rugosin D

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