Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing

Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing

Resistance of gRNA to ubiquitous ribonucleases is required for CRISPR-Cas9-based therapeutics. Here, the authors explore chemical modifications at all positions of the crRNA guide and tracrRNA cofactor, and identify modified versions that are more potent and stable than their unmodified counterparts...

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Bibliographic Details
Main Authors: Aamir Mir, Julia F. Alterman, Matthew R. Hassler, Alexandre J. Debacker, Edward Hudgens, Dimas Echeverria, Michael H. Brodsky, Anastasia Khvorova, Jonathan K. Watts, Erik J. Sontheimer
Format: article
Language:EN
Published: Nature Portfolio 2018
Subjects:
Science
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Online Access:https://doaj.org/article/b945b146d35544ef9d5a4a30d633c3c0
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