The flavonoid apigenin downregulates CDK1 by directly targeting ribosomal protein S9.

Flavonoids have been reported to inhibit tumor growth by causing cell cycle arrest. However, little is known about the direct targets of flavonoids in tumor growth inhibition. In the present study, we developed a novel method using magnetic FG beads to purify flavonoid-binding proteins, and identifi...

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Autores principales:	Yosuke Iizumi, Masakatsu Oishi, Tomoyuki Taniguchi, Wakana Goi, Yoshihiro Sowa, Toshiyuki Sakai
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ba9f369344d24d1698b1994292a9877e
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spelling	oai:doaj.org-article:ba9f369344d24d1698b1994292a9877e2021-11-18T08:57:42ZThe flavonoid apigenin downregulates CDK1 by directly targeting ribosomal protein S9.1932-620310.1371/journal.pone.0073219https://doaj.org/article/ba9f369344d24d1698b1994292a9877e2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24009741/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Flavonoids have been reported to inhibit tumor growth by causing cell cycle arrest. However, little is known about the direct targets of flavonoids in tumor growth inhibition. In the present study, we developed a novel method using magnetic FG beads to purify flavonoid-binding proteins, and identified ribosomal protein S9 (RPS9) as a binding partner of the flavonoid apigenin. Similar to treatment with apigenin, knockdown of RPS9 inhibited the growth of human colon cancer cells at the G2/M phase by downregulating cyclin-dependent kinase 1 (CDK1) expression at the promoter level. Furthermore, knockdown of RPS9 suppressed G2/M arrest caused by apigenin. These results suggest that apigenin induces G2/M arrest at least partially by directly binding and inhibiting RPS9 which enhances CDK1 expression. We therefore raise the possibility that identification of the direct targets of flavonoids may contribute to the discovery of novel molecular mechanisms governing tumor growth.Yosuke IizumiMasakatsu OishiTomoyuki TaniguchiWakana GoiYoshihiro SowaToshiyuki SakaiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e73219 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yosuke Iizumi Masakatsu Oishi Tomoyuki Taniguchi Wakana Goi Yoshihiro Sowa Toshiyuki Sakai The flavonoid apigenin downregulates CDK1 by directly targeting ribosomal protein S9.
description	Flavonoids have been reported to inhibit tumor growth by causing cell cycle arrest. However, little is known about the direct targets of flavonoids in tumor growth inhibition. In the present study, we developed a novel method using magnetic FG beads to purify flavonoid-binding proteins, and identified ribosomal protein S9 (RPS9) as a binding partner of the flavonoid apigenin. Similar to treatment with apigenin, knockdown of RPS9 inhibited the growth of human colon cancer cells at the G2/M phase by downregulating cyclin-dependent kinase 1 (CDK1) expression at the promoter level. Furthermore, knockdown of RPS9 suppressed G2/M arrest caused by apigenin. These results suggest that apigenin induces G2/M arrest at least partially by directly binding and inhibiting RPS9 which enhances CDK1 expression. We therefore raise the possibility that identification of the direct targets of flavonoids may contribute to the discovery of novel molecular mechanisms governing tumor growth.
format	article
author	Yosuke Iizumi Masakatsu Oishi Tomoyuki Taniguchi Wakana Goi Yoshihiro Sowa Toshiyuki Sakai
author_facet	Yosuke Iizumi Masakatsu Oishi Tomoyuki Taniguchi Wakana Goi Yoshihiro Sowa Toshiyuki Sakai
author_sort	Yosuke Iizumi
title	The flavonoid apigenin downregulates CDK1 by directly targeting ribosomal protein S9.
title_short	The flavonoid apigenin downregulates CDK1 by directly targeting ribosomal protein S9.
title_full	The flavonoid apigenin downregulates CDK1 by directly targeting ribosomal protein S9.
title_fullStr	The flavonoid apigenin downregulates CDK1 by directly targeting ribosomal protein S9.
title_full_unstemmed	The flavonoid apigenin downregulates CDK1 by directly targeting ribosomal protein S9.
title_sort	flavonoid apigenin downregulates cdk1 by directly targeting ribosomal protein s9.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ba9f369344d24d1698b1994292a9877e
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The flavonoid apigenin downregulates CDK1 by directly targeting ribosomal protein S9.

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