Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens

Phytopathogenic fungal growth in postharvest fruits and vegetables is responsible for 20–25% of production losses. Volatile organic compounds (VOCs) have been gaining importance in the food industry as a safe and ecofriendly alternative to pesticides for combating these phytopathogenic fungi. In thi...

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Autores principales:	Laura Toral, Miguel Rodríguez, Fernando Martínez-Checa, Alfredo Montaño, Amparo Cortés-Delgado, Agnieszka Smolinska, Inmaculada Llamas, Inmaculada Sampedro
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	volatile compounds antifungal activity biocontrol fungal phytopathogens postharvest diseases Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/badfee66dc9746a19dd6425e1ba946a9
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spelling	oai:doaj.org-article:badfee66dc9746a19dd6425e1ba946a92021-11-12T05:55:57ZIdentification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens1664-302X10.3389/fmicb.2021.773092https://doaj.org/article/badfee66dc9746a19dd6425e1ba946a92021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.773092/fullhttps://doaj.org/toc/1664-302XPhytopathogenic fungal growth in postharvest fruits and vegetables is responsible for 20–25% of production losses. Volatile organic compounds (VOCs) have been gaining importance in the food industry as a safe and ecofriendly alternative to pesticides for combating these phytopathogenic fungi. In this study, we analysed the ability of some VOCs produced by strains of the genera Bacillus, Peribacillus, Pseudomonas, Psychrobacillus and Staphylococcus to inhibit the growth of Alternaria alternata, Botrytis cinerea, Fusarium oxysporum, Fusarium solani, Monilinia fructicola, Monilinia laxa and Sclerotinia sclerotiorum, in vitro and in vivo. We analysed bacterial VOCs by using GC/MS and 87 volatile compounds were identified, in particular acetoin, acetic acid, 2,3-butanediol, isopentanol, dimethyl disulphide and isopentyl isobutanoate. In vitro growth inhibition assays and in vivo experiments using cherry fruits showed that the best producers of VOCs, Bacillus atrophaeus L193, Bacillus velezensis XT1 and Psychrobacillus vulpis Z8, exhibited the highest antifungal activity against B. cinerea, M. fructicola and M. laxa, which highlights the potential of these strains to control postharvest diseases. Transmission electron microscopy micrographs of bacterial VOC-treated fungi clearly showed antifungal activity which led to an intense degeneration of cellular components of mycelium and cell death.Laura ToralMiguel RodríguezMiguel RodríguezFernando Martínez-ChecaFernando Martínez-ChecaAlfredo MontañoAmparo Cortés-DelgadoAgnieszka SmolinskaInmaculada LlamasInmaculada LlamasInmaculada SampedroInmaculada SampedroFrontiers Media S.A.articlevolatile compoundsantifungal activitybiocontrolfungal phytopathogenspostharvest diseasesMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	volatile compounds antifungal activity biocontrol fungal phytopathogens postharvest diseases Microbiology QR1-502
spellingShingle	volatile compounds antifungal activity biocontrol fungal phytopathogens postharvest diseases Microbiology QR1-502 Laura Toral Miguel Rodríguez Miguel Rodríguez Fernando Martínez-Checa Fernando Martínez-Checa Alfredo Montaño Amparo Cortés-Delgado Agnieszka Smolinska Inmaculada Llamas Inmaculada Llamas Inmaculada Sampedro Inmaculada Sampedro Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens
description	Phytopathogenic fungal growth in postharvest fruits and vegetables is responsible for 20–25% of production losses. Volatile organic compounds (VOCs) have been gaining importance in the food industry as a safe and ecofriendly alternative to pesticides for combating these phytopathogenic fungi. In this study, we analysed the ability of some VOCs produced by strains of the genera Bacillus, Peribacillus, Pseudomonas, Psychrobacillus and Staphylococcus to inhibit the growth of Alternaria alternata, Botrytis cinerea, Fusarium oxysporum, Fusarium solani, Monilinia fructicola, Monilinia laxa and Sclerotinia sclerotiorum, in vitro and in vivo. We analysed bacterial VOCs by using GC/MS and 87 volatile compounds were identified, in particular acetoin, acetic acid, 2,3-butanediol, isopentanol, dimethyl disulphide and isopentyl isobutanoate. In vitro growth inhibition assays and in vivo experiments using cherry fruits showed that the best producers of VOCs, Bacillus atrophaeus L193, Bacillus velezensis XT1 and Psychrobacillus vulpis Z8, exhibited the highest antifungal activity against B. cinerea, M. fructicola and M. laxa, which highlights the potential of these strains to control postharvest diseases. Transmission electron microscopy micrographs of bacterial VOC-treated fungi clearly showed antifungal activity which led to an intense degeneration of cellular components of mycelium and cell death.
format	article
author	Laura Toral Miguel Rodríguez Miguel Rodríguez Fernando Martínez-Checa Fernando Martínez-Checa Alfredo Montaño Amparo Cortés-Delgado Agnieszka Smolinska Inmaculada Llamas Inmaculada Llamas Inmaculada Sampedro Inmaculada Sampedro
author_facet	Laura Toral Miguel Rodríguez Miguel Rodríguez Fernando Martínez-Checa Fernando Martínez-Checa Alfredo Montaño Amparo Cortés-Delgado Agnieszka Smolinska Inmaculada Llamas Inmaculada Llamas Inmaculada Sampedro Inmaculada Sampedro
author_sort	Laura Toral
title	Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens
title_short	Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens
title_full	Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens
title_fullStr	Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens
title_full_unstemmed	Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens
title_sort	identification of volatile organic compounds in extremophilic bacteria and their effective use in biocontrol of postharvest fungal phytopathogens
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/badfee66dc9746a19dd6425e1ba946a9
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Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens

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