Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries

Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries

Existing Förster Resonance Energy Transfer (FRET) biosensors are often limited in their sensitivity. Here the authors report FRET-seq which they use to identify Fyn and ZAP70 kinase biosensors with enhanced performance, and use them to image T-cell activation and screen drugs.

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Bibliographic Details
Main Authors: Longwei Liu, Praopim Limsakul, Xianhui Meng, Yan Huang, Reed E. S. Harrison, Tse-Shun Huang, Yiwen Shi, Yiyan Yu, Krit Charupanit, Sheng Zhong, Shaoying Lu, Jin Zhang, Shu Chien, Jie Sun, Yingxiao Wang
Format: article
Language:EN
Published: Nature Portfolio 2021
Subjects:
Science
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Online Access:https://doaj.org/article/bb6ff210871349e99294dd89352b8506
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Internet

https://doaj.org/article/bb6ff210871349e99294dd89352b8506

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