Correlation Analysis of Antioxidant Activities with Tannin, Total Flavonoid, and Total Phenolic Contents of Nutmeg (Myristica fragrans Houtt) Fruit Precipitated by Egg white

The nutmeg (Myristica fragrans) flesh extract has a strong antioxidant activity. Therefore, M. fragrans flesh can be developed for functional drinks which are sources rich in antioxidants good for the prevention and treatment of diseases such as cancer and cardiovascular diseases. However, the tanni...

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Autores principales:	Irma Antasionasti, Olvie Syenni Datu, Utami Sasmita Lestari, Surya Sumantri Abdullah, Imam Jayanto
Formato:	article
Lenguaje:	EN ID
Publicado:	Institute for Researches and Community Services Universitas Muhammadiyah Palangkaraya 2021
Materias:	Nutmeg flesh extract DPPH ABTS Total flavonoid content Total phenolic content Tannin content Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/bbb3c177a2ed491687155a17497d78da
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spelling	oai:doaj.org-article:bbb3c177a2ed491687155a17497d78da2021-12-01T04:44:27ZCorrelation Analysis of Antioxidant Activities with Tannin, Total Flavonoid, and Total Phenolic Contents of Nutmeg (Myristica fragrans Houtt) Fruit Precipitated by Egg white10.33084/bjop.v4i4.24972621-4814https://doaj.org/article/bbb3c177a2ed491687155a17497d78da2021-11-01T00:00:00Zhttp://journal.umpalangkaraya.ac.id/index.php/bjop/article/view/2497https://doaj.org/toc/2621-4814The nutmeg (Myristica fragrans) flesh extract has a strong antioxidant activity. Therefore, M. fragrans flesh can be developed for functional drinks which are sources rich in antioxidants good for the prevention and treatment of diseases such as cancer and cardiovascular diseases. However, the tannins' content can cause a bitter and sour taste. Therefore, the tannins content should be reduced by the addition of egg white. The purpose of this study is to find out the comparison of antioxidant activity between a combination of M. fragrans flesh extract with various concentrations of egg white and to correlate its antioxidant activities with tannin, total flavonoid, and total phenolic contents. The antioxidant activities were conducted on M. fragrans flesh extract by using DPPH and ABTS radicals. Tannin, total flavonoid, and total phenolic contents from M. fragrans flesh extract were also tested. The M. fragrans flesh extracts without addition egg white have a strong antioxidant in scavenging the stable free radical ABTS (89.980±0.480 µg/mL) and intermediate antioxidant in scavenging the stable free radical DPPH (105.669±0.102 µg/mL). It is followed accordingly by tannin, total flavonoid, and total phenolic contents, namely 14.034±0.100 %w/w TAE, 26.929±0.129 %w/w QE, and 53.164±0.129 %w/w GAE, respectively. Correlation of tannin, total flavonoid, and total phenolic contents, which inhibited DPPH and ABTS radicals had R2 values of about 89.23-97.63%. It showed that antioxidant activity is strongly influenced by the tannin, total flavonoid, and total phenolic contents. Therefore, precipitation from the tannin-protein bond caused antioxidant activities were decreased. Irma AntasionastiOlvie Syenni DatuUtami Sasmita LestariSurya Sumantri AbdullahImam JayantoInstitute for Researches and Community Services Universitas Muhammadiyah Palangkaraya articleNutmeg flesh extractDPPHABTSTotal flavonoid contentTotal phenolic contentTannin contentPharmacy and materia medicaRS1-441ENIDBorneo Journal of Pharmacy, Vol 4, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	Nutmeg flesh extract DPPH ABTS Total flavonoid content Total phenolic content Tannin content Pharmacy and materia medica RS1-441
spellingShingle	Nutmeg flesh extract DPPH ABTS Total flavonoid content Total phenolic content Tannin content Pharmacy and materia medica RS1-441 Irma Antasionasti Olvie Syenni Datu Utami Sasmita Lestari Surya Sumantri Abdullah Imam Jayanto Correlation Analysis of Antioxidant Activities with Tannin, Total Flavonoid, and Total Phenolic Contents of Nutmeg (Myristica fragrans Houtt) Fruit Precipitated by Egg white
description	The nutmeg (Myristica fragrans) flesh extract has a strong antioxidant activity. Therefore, M. fragrans flesh can be developed for functional drinks which are sources rich in antioxidants good for the prevention and treatment of diseases such as cancer and cardiovascular diseases. However, the tannins' content can cause a bitter and sour taste. Therefore, the tannins content should be reduced by the addition of egg white. The purpose of this study is to find out the comparison of antioxidant activity between a combination of M. fragrans flesh extract with various concentrations of egg white and to correlate its antioxidant activities with tannin, total flavonoid, and total phenolic contents. The antioxidant activities were conducted on M. fragrans flesh extract by using DPPH and ABTS radicals. Tannin, total flavonoid, and total phenolic contents from M. fragrans flesh extract were also tested. The M. fragrans flesh extracts without addition egg white have a strong antioxidant in scavenging the stable free radical ABTS (89.980±0.480 µg/mL) and intermediate antioxidant in scavenging the stable free radical DPPH (105.669±0.102 µg/mL). It is followed accordingly by tannin, total flavonoid, and total phenolic contents, namely 14.034±0.100 %w/w TAE, 26.929±0.129 %w/w QE, and 53.164±0.129 %w/w GAE, respectively. Correlation of tannin, total flavonoid, and total phenolic contents, which inhibited DPPH and ABTS radicals had R2 values of about 89.23-97.63%. It showed that antioxidant activity is strongly influenced by the tannin, total flavonoid, and total phenolic contents. Therefore, precipitation from the tannin-protein bond caused antioxidant activities were decreased.
format	article
author	Irma Antasionasti Olvie Syenni Datu Utami Sasmita Lestari Surya Sumantri Abdullah Imam Jayanto
author_facet	Irma Antasionasti Olvie Syenni Datu Utami Sasmita Lestari Surya Sumantri Abdullah Imam Jayanto
author_sort	Irma Antasionasti
title	Correlation Analysis of Antioxidant Activities with Tannin, Total Flavonoid, and Total Phenolic Contents of Nutmeg (Myristica fragrans Houtt) Fruit Precipitated by Egg white
title_short	Correlation Analysis of Antioxidant Activities with Tannin, Total Flavonoid, and Total Phenolic Contents of Nutmeg (Myristica fragrans Houtt) Fruit Precipitated by Egg white
title_full	Correlation Analysis of Antioxidant Activities with Tannin, Total Flavonoid, and Total Phenolic Contents of Nutmeg (Myristica fragrans Houtt) Fruit Precipitated by Egg white
title_fullStr	Correlation Analysis of Antioxidant Activities with Tannin, Total Flavonoid, and Total Phenolic Contents of Nutmeg (Myristica fragrans Houtt) Fruit Precipitated by Egg white
title_full_unstemmed	Correlation Analysis of Antioxidant Activities with Tannin, Total Flavonoid, and Total Phenolic Contents of Nutmeg (Myristica fragrans Houtt) Fruit Precipitated by Egg white
title_sort	correlation analysis of antioxidant activities with tannin, total flavonoid, and total phenolic contents of nutmeg (myristica fragrans houtt) fruit precipitated by egg white
publisher	Institute for Researches and Community Services Universitas Muhammadiyah Palangkaraya
publishDate	2021
url	https://doaj.org/article/bbb3c177a2ed491687155a17497d78da
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Correlation Analysis of Antioxidant Activities with Tannin, Total Flavonoid, and Total Phenolic Contents of Nutmeg (Myristica fragrans Houtt) Fruit Precipitated by Egg white

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