Cell fate clusters in ICM organoids arise from cell fate heredity and division: a modelling approach

Abstract During the mammalian preimplantation phase, cells undergo two subsequent cell fate decisions. During the first decision, the trophectoderm and the inner cell mass are formed. Subsequently, the inner cell mass segregates into the epiblast and the primitive endoderm. Inner cell mass organoids...

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Autores principales:	Tim Liebisch, Armin Drusko, Biena Mathew, Ernst H. K. Stelzer, Sabine C. Fischer, Franziska Matthäus
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc322e20319b4056bfa3b840aeedbfc9
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spelling	oai:doaj.org-article:bc322e20319b4056bfa3b840aeedbfc92021-12-02T14:01:27ZCell fate clusters in ICM organoids arise from cell fate heredity and division: a modelling approach10.1038/s41598-020-80141-32045-2322https://doaj.org/article/bc322e20319b4056bfa3b840aeedbfc92020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80141-3https://doaj.org/toc/2045-2322Abstract During the mammalian preimplantation phase, cells undergo two subsequent cell fate decisions. During the first decision, the trophectoderm and the inner cell mass are formed. Subsequently, the inner cell mass segregates into the epiblast and the primitive endoderm. Inner cell mass organoids represent an experimental model system, mimicking the second cell fate decision. It has been shown that cells of the same fate tend to cluster stronger than expected for random cell fate decisions. Three major processes are hypothesised to contribute to the cell fate arrangements: (1) chemical signalling; (2) cell sorting; and (3) cell proliferation. In order to quantify the influence of cell proliferation on the observed cell lineage type clustering, we developed an agent-based model accounting for mechanical cell–cell interaction, i.e. adhesion and repulsion, cell division, stochastic cell fate decision and cell fate heredity. The model supports the hypothesis that initial cell fate acquisition is a stochastically driven process, taking place in the early development of inner cell mass organoids. Further, we show that the observed neighbourhood structures can emerge solely due to cell fate heredity during cell division.Tim LiebischArmin DruskoBiena MathewErnst H. K. StelzerSabine C. FischerFranziska MatthäusNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tim Liebisch Armin Drusko Biena Mathew Ernst H. K. Stelzer Sabine C. Fischer Franziska Matthäus Cell fate clusters in ICM organoids arise from cell fate heredity and division: a modelling approach
description	Abstract During the mammalian preimplantation phase, cells undergo two subsequent cell fate decisions. During the first decision, the trophectoderm and the inner cell mass are formed. Subsequently, the inner cell mass segregates into the epiblast and the primitive endoderm. Inner cell mass organoids represent an experimental model system, mimicking the second cell fate decision. It has been shown that cells of the same fate tend to cluster stronger than expected for random cell fate decisions. Three major processes are hypothesised to contribute to the cell fate arrangements: (1) chemical signalling; (2) cell sorting; and (3) cell proliferation. In order to quantify the influence of cell proliferation on the observed cell lineage type clustering, we developed an agent-based model accounting for mechanical cell–cell interaction, i.e. adhesion and repulsion, cell division, stochastic cell fate decision and cell fate heredity. The model supports the hypothesis that initial cell fate acquisition is a stochastically driven process, taking place in the early development of inner cell mass organoids. Further, we show that the observed neighbourhood structures can emerge solely due to cell fate heredity during cell division.
format	article
author	Tim Liebisch Armin Drusko Biena Mathew Ernst H. K. Stelzer Sabine C. Fischer Franziska Matthäus
author_facet	Tim Liebisch Armin Drusko Biena Mathew Ernst H. K. Stelzer Sabine C. Fischer Franziska Matthäus
author_sort	Tim Liebisch
title	Cell fate clusters in ICM organoids arise from cell fate heredity and division: a modelling approach
title_short	Cell fate clusters in ICM organoids arise from cell fate heredity and division: a modelling approach
title_full	Cell fate clusters in ICM organoids arise from cell fate heredity and division: a modelling approach
title_fullStr	Cell fate clusters in ICM organoids arise from cell fate heredity and division: a modelling approach
title_full_unstemmed	Cell fate clusters in ICM organoids arise from cell fate heredity and division: a modelling approach
title_sort	cell fate clusters in icm organoids arise from cell fate heredity and division: a modelling approach
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/bc322e20319b4056bfa3b840aeedbfc9
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_version_	1718392165548163072

Cell fate clusters in ICM organoids arise from cell fate heredity and division: a modelling approach

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