Deciphering the scopolamine challenge rat model by preclinical functional MRI

Abstract During preclinical drug testing, the systemic administration of scopolamine (SCO), a cholinergic antagonist, is widely used. However, it suffers important limitations, like non-specific behavioural effects partly due to its peripheral side-effects. Therefore, neuroimaging measures would enh...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gergely Somogyi, Dávid Hlatky, Tamás Spisák, Zsófia Spisák, Gabriella Nyitrai, András Czurkó
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bc375befec4241b7a5e689a909becee5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bc375befec4241b7a5e689a909becee5
record_format	dspace
spelling	oai:doaj.org-article:bc375befec4241b7a5e689a909becee52021-12-02T14:49:10ZDeciphering the scopolamine challenge rat model by preclinical functional MRI10.1038/s41598-021-90273-92045-2322https://doaj.org/article/bc375befec4241b7a5e689a909becee52021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90273-9https://doaj.org/toc/2045-2322Abstract During preclinical drug testing, the systemic administration of scopolamine (SCO), a cholinergic antagonist, is widely used. However, it suffers important limitations, like non-specific behavioural effects partly due to its peripheral side-effects. Therefore, neuroimaging measures would enhance its translational value. To this end, in Wistar rats, we measured whisker-stimulation induced functional MRI activation after SCO, peripherally acting butylscopolamine (BSCO), or saline administration in a cross-over design. Besides the commonly used gradient-echo echo-planar imaging (GE EPI), we also used an arterial spin labeling method in isoflurane anesthesia. With the GE EPI measurement, SCO decreased the evoked BOLD response in the barrel cortex (BC), while BSCO increased it in the anterior cingulate cortex. In a second experiment, we used GE EPI and spin-echo (SE) EPI sequences in a combined (isoflurane + i.p. dexmedetomidine) anesthesia to account for anesthesia-effects. Here, we also examined the effect of donepezil. In the combined anesthesia, with the GE EPI, SCO decreased the activation in the BC and the inferior colliculus (IC). BSCO reduced the response merely in the IC. Our results revealed that SCO attenuated the evoked BOLD activation in the BC as a probable central effect in both experiments. The likely peripheral vascular actions of SCO with the given fMRI sequences depended on the type of anesthesia or its dose.Gergely SomogyiDávid HlatkyTamás SpisákZsófia SpisákGabriella NyitraiAndrás CzurkóNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gergely Somogyi Dávid Hlatky Tamás Spisák Zsófia Spisák Gabriella Nyitrai András Czurkó Deciphering the scopolamine challenge rat model by preclinical functional MRI
description	Abstract During preclinical drug testing, the systemic administration of scopolamine (SCO), a cholinergic antagonist, is widely used. However, it suffers important limitations, like non-specific behavioural effects partly due to its peripheral side-effects. Therefore, neuroimaging measures would enhance its translational value. To this end, in Wistar rats, we measured whisker-stimulation induced functional MRI activation after SCO, peripherally acting butylscopolamine (BSCO), or saline administration in a cross-over design. Besides the commonly used gradient-echo echo-planar imaging (GE EPI), we also used an arterial spin labeling method in isoflurane anesthesia. With the GE EPI measurement, SCO decreased the evoked BOLD response in the barrel cortex (BC), while BSCO increased it in the anterior cingulate cortex. In a second experiment, we used GE EPI and spin-echo (SE) EPI sequences in a combined (isoflurane + i.p. dexmedetomidine) anesthesia to account for anesthesia-effects. Here, we also examined the effect of donepezil. In the combined anesthesia, with the GE EPI, SCO decreased the activation in the BC and the inferior colliculus (IC). BSCO reduced the response merely in the IC. Our results revealed that SCO attenuated the evoked BOLD activation in the BC as a probable central effect in both experiments. The likely peripheral vascular actions of SCO with the given fMRI sequences depended on the type of anesthesia or its dose.
format	article
author	Gergely Somogyi Dávid Hlatky Tamás Spisák Zsófia Spisák Gabriella Nyitrai András Czurkó
author_facet	Gergely Somogyi Dávid Hlatky Tamás Spisák Zsófia Spisák Gabriella Nyitrai András Czurkó
author_sort	Gergely Somogyi
title	Deciphering the scopolamine challenge rat model by preclinical functional MRI
title_short	Deciphering the scopolamine challenge rat model by preclinical functional MRI
title_full	Deciphering the scopolamine challenge rat model by preclinical functional MRI
title_fullStr	Deciphering the scopolamine challenge rat model by preclinical functional MRI
title_full_unstemmed	Deciphering the scopolamine challenge rat model by preclinical functional MRI
title_sort	deciphering the scopolamine challenge rat model by preclinical functional mri
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bc375befec4241b7a5e689a909becee5
work_keys_str_mv	AT gergelysomogyi decipheringthescopolaminechallengeratmodelbypreclinicalfunctionalmri AT davidhlatky decipheringthescopolaminechallengeratmodelbypreclinicalfunctionalmri AT tamasspisak decipheringthescopolaminechallengeratmodelbypreclinicalfunctionalmri AT zsofiaspisak decipheringthescopolaminechallengeratmodelbypreclinicalfunctionalmri AT gabriellanyitrai decipheringthescopolaminechallengeratmodelbypreclinicalfunctionalmri AT andrasczurko decipheringthescopolaminechallengeratmodelbypreclinicalfunctionalmri
_version_	1718389531945730048

Deciphering the scopolamine challenge rat model by preclinical functional MRI

Ejemplares similares