dCas9 regulator to neutralize competition in CRISPRi circuits

CRISPRi allows for the simultaneous control of many genes, however the sgRNAs compete for binding to dCas9. Here the authors design a dCas9 concentration regulator to allow independent regulation of multiple genes.

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Auteurs principaux: Hsin-Ho Huang, Massimo Bellato, Yili Qian, Pablo Cárdenas, Lorenzo Pasotti, Paolo Magni, Domitilla Del Vecchio
Format: article
Langue:EN
Publié: Nature Portfolio 2021
Sujets:
Q
Accès en ligne:https://doaj.org/article/bc77626575304f0082d2047b7dc45282
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