dCas9 regulator to neutralize competition in CRISPRi circuits

CRISPRi allows for the simultaneous control of many genes, however the sgRNAs compete for binding to dCas9. Here the authors design a dCas9 concentration regulator to allow independent regulation of multiple genes.

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Autores principales:	Hsin-Ho Huang, Massimo Bellato, Yili Qian, Pablo Cárdenas, Lorenzo Pasotti, Paolo Magni, Domitilla Del Vecchio
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/bc77626575304f0082d2047b7dc45282
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spelling	oai:doaj.org-article:bc77626575304f0082d2047b7dc452822021-12-02T17:05:49ZdCas9 regulator to neutralize competition in CRISPRi circuits10.1038/s41467-021-21772-62041-1723https://doaj.org/article/bc77626575304f0082d2047b7dc452822021-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21772-6https://doaj.org/toc/2041-1723CRISPRi allows for the simultaneous control of many genes, however the sgRNAs compete for binding to dCas9. Here the authors design a dCas9 concentration regulator to allow independent regulation of multiple genes.Hsin-Ho HuangMassimo BellatoYili QianPablo CárdenasLorenzo PasottiPaolo MagniDomitilla Del VecchioNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Hsin-Ho Huang Massimo Bellato Yili Qian Pablo Cárdenas Lorenzo Pasotti Paolo Magni Domitilla Del Vecchio dCas9 regulator to neutralize competition in CRISPRi circuits
description	CRISPRi allows for the simultaneous control of many genes, however the sgRNAs compete for binding to dCas9. Here the authors design a dCas9 concentration regulator to allow independent regulation of multiple genes.
format	article
author	Hsin-Ho Huang Massimo Bellato Yili Qian Pablo Cárdenas Lorenzo Pasotti Paolo Magni Domitilla Del Vecchio
author_facet	Hsin-Ho Huang Massimo Bellato Yili Qian Pablo Cárdenas Lorenzo Pasotti Paolo Magni Domitilla Del Vecchio
author_sort	Hsin-Ho Huang
title	dCas9 regulator to neutralize competition in CRISPRi circuits
title_short	dCas9 regulator to neutralize competition in CRISPRi circuits
title_full	dCas9 regulator to neutralize competition in CRISPRi circuits
title_fullStr	dCas9 regulator to neutralize competition in CRISPRi circuits
title_full_unstemmed	dCas9 regulator to neutralize competition in CRISPRi circuits
title_sort	dcas9 regulator to neutralize competition in crispri circuits
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bc77626575304f0082d2047b7dc45282
work_keys_str_mv	AT hsinhohuang dcas9regulatortoneutralizecompetitionincrispricircuits AT massimobellato dcas9regulatortoneutralizecompetitionincrispricircuits AT yiliqian dcas9regulatortoneutralizecompetitionincrispricircuits AT pablocardenas dcas9regulatortoneutralizecompetitionincrispricircuits AT lorenzopasotti dcas9regulatortoneutralizecompetitionincrispricircuits AT paolomagni dcas9regulatortoneutralizecompetitionincrispricircuits AT domitilladelvecchio dcas9regulatortoneutralizecompetitionincrispricircuits
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dCas9 regulator to neutralize competition in CRISPRi circuits

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