Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease

In bacteria, CRISPR-Cas9 identifies and cleaves the target DNA with the assistance of a tracrRNA and a crRNA. Here the authors use single-molecule spectroscopy to investigate conformational changes and show that the tracrRNA keeps Cas9 in a functionally active form.

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Main Authors:	Youngbin Lim, So Young Bak, Keewon Sung, Euihwan Jeong, Seung Hwan Lee, Jin-Soo Kim, Sangsu Bae, Seong Keun Kim
Format:	article
Language:	EN
Published:	Nature Portfolio 2016
Subjects:	Science Q
Online Access:	https://doaj.org/article/bd61a1e9e4df45b785666f33100ca9fe
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spelling	oai:doaj.org-article:bd61a1e9e4df45b785666f33100ca9fe2021-12-02T15:35:46ZStructural roles of guide RNAs in the nuclease activity of Cas9 endonuclease10.1038/ncomms133502041-1723https://doaj.org/article/bd61a1e9e4df45b785666f33100ca9fe2016-11-01T00:00:00Zhttps://doi.org/10.1038/ncomms13350https://doaj.org/toc/2041-1723In bacteria, CRISPR-Cas9 identifies and cleaves the target DNA with the assistance of a tracrRNA and a crRNA. Here the authors use single-molecule spectroscopy to investigate conformational changes and show that the tracrRNA keeps Cas9 in a functionally active form.Youngbin LimSo Young BakKeewon SungEuihwan JeongSeung Hwan LeeJin-Soo KimSangsu BaeSeong Keun KimNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-8 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Youngbin Lim So Young Bak Keewon Sung Euihwan Jeong Seung Hwan Lee Jin-Soo Kim Sangsu Bae Seong Keun Kim Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease
description	In bacteria, CRISPR-Cas9 identifies and cleaves the target DNA with the assistance of a tracrRNA and a crRNA. Here the authors use single-molecule spectroscopy to investigate conformational changes and show that the tracrRNA keeps Cas9 in a functionally active form.
format	article
author	Youngbin Lim So Young Bak Keewon Sung Euihwan Jeong Seung Hwan Lee Jin-Soo Kim Sangsu Bae Seong Keun Kim
author_facet	Youngbin Lim So Young Bak Keewon Sung Euihwan Jeong Seung Hwan Lee Jin-Soo Kim Sangsu Bae Seong Keun Kim
author_sort	Youngbin Lim
title	Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease
title_short	Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease
title_full	Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease
title_fullStr	Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease
title_full_unstemmed	Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease
title_sort	structural roles of guide rnas in the nuclease activity of cas9 endonuclease
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/bd61a1e9e4df45b785666f33100ca9fe
work_keys_str_mv	AT youngbinlim structuralrolesofguidernasinthenucleaseactivityofcas9endonuclease AT soyoungbak structuralrolesofguidernasinthenucleaseactivityofcas9endonuclease AT keewonsung structuralrolesofguidernasinthenucleaseactivityofcas9endonuclease AT euihwanjeong structuralrolesofguidernasinthenucleaseactivityofcas9endonuclease AT seunghwanlee structuralrolesofguidernasinthenucleaseactivityofcas9endonuclease AT jinsookim structuralrolesofguidernasinthenucleaseactivityofcas9endonuclease AT sangsubae structuralrolesofguidernasinthenucleaseactivityofcas9endonuclease AT seongkeunkim structuralrolesofguidernasinthenucleaseactivityofcas9endonuclease
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Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease

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