Improving the Precision of Base Editing by Bubble Hairpin Single Guide RNA

Base editors are double-strand-break (DSB)-free genome editing tools and have been widely used in diverse living systems. However, it is reported that these tools can cause substantial off-target editing.

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Detalles Bibliográficos
Autores principales: Zhiwei Hu, Yannan Wang, Qian Liu, Yan Qiu, Zhiyu Zhong, Kangdi Li, Wenhua Li, Zixin Deng, Yuhui Sun
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2021
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Acceso en línea:https://doaj.org/article/bdac9787d38e4570b75d966420a1da21
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Descripción
Sumario:Base editors are double-strand-break (DSB)-free genome editing tools and have been widely used in diverse living systems. However, it is reported that these tools can cause substantial off-target editing.