Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions

Most approaches to control gene expression in vivo require generation of knock-in mouse lines and often lack spatiotemporal control. Here the authors develop a photo-activatable Flp recombinase system and demonstrate its use by controlling object-exploration behavior in mice through Cav3.1 silencing...

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Autores principales:	Hyunjin Jung, Seong-Wook Kim, Minsoo Kim, Jongryul Hong, Daseuli Yu, Ji Hye Kim, Yunju Lee, Sungsoo Kim, Doyeon Woo, Hee-Sup Shin, Byung Ouk Park, Won Do Heo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/bdb35816507e4ab195f71dcd83e79b7e
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spelling	oai:doaj.org-article:bdb35816507e4ab195f71dcd83e79b7e2021-12-02T14:38:46ZNoninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions10.1038/s41467-018-08282-82041-1723https://doaj.org/article/bdb35816507e4ab195f71dcd83e79b7e2019-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-08282-8https://doaj.org/toc/2041-1723Most approaches to control gene expression in vivo require generation of knock-in mouse lines and often lack spatiotemporal control. Here the authors develop a photo-activatable Flp recombinase system and demonstrate its use by controlling object-exploration behavior in mice through Cav3.1 silencing.Hyunjin JungSeong-Wook KimMinsoo KimJongryul HongDaseuli YuJi Hye KimYunju LeeSungsoo KimDoyeon WooHee-Sup ShinByung Ouk ParkWon Do HeoNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Hyunjin Jung Seong-Wook Kim Minsoo Kim Jongryul Hong Daseuli Yu Ji Hye Kim Yunju Lee Sungsoo Kim Doyeon Woo Hee-Sup Shin Byung Ouk Park Won Do Heo Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions
description	Most approaches to control gene expression in vivo require generation of knock-in mouse lines and often lack spatiotemporal control. Here the authors develop a photo-activatable Flp recombinase system and demonstrate its use by controlling object-exploration behavior in mice through Cav3.1 silencing.
format	article
author	Hyunjin Jung Seong-Wook Kim Minsoo Kim Jongryul Hong Daseuli Yu Ji Hye Kim Yunju Lee Sungsoo Kim Doyeon Woo Hee-Sup Shin Byung Ouk Park Won Do Heo
author_facet	Hyunjin Jung Seong-Wook Kim Minsoo Kim Jongryul Hong Daseuli Yu Ji Hye Kim Yunju Lee Sungsoo Kim Doyeon Woo Hee-Sup Shin Byung Ouk Park Won Do Heo
author_sort	Hyunjin Jung
title	Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions
title_short	Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions
title_full	Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions
title_fullStr	Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions
title_full_unstemmed	Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions
title_sort	noninvasive optical activation of flp recombinase for genetic manipulation in deep mouse brain regions
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/bdb35816507e4ab195f71dcd83e79b7e
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Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions

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