Mixed <named-content content-type="genus-species">Chlamydia trachomatis</named-content> Peptide Antigens Provide a Specific and Sensitive Single-Well Colorimetric Enzyme-Linked Immunosorbent Assay for Detection of Human Anti<italic toggle="yes">-</italic><named-content content-type="genus-species">C. trachomatis</named-content> Antibodies

ABSTRACT Sensitive and specific detection of anti-Chlamydia trachomatis antibodies in standard enzyme-linked immunosorbent assays (ELISAs) is compromised by cross-reactivity and poor sensitivity of classical C. trachomatis antigens. Previously, we discovered 48 strongly reactive peptide antigens of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	K. Shamsur Rahman, Toni Darville, Harold C. Wiesenfeld, Sharon L. Hillier, Bernhard Kaltenboeck
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Chlamydia trachomatis diagnosis serology antibody detection B-cell epitopes peptide antigens Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/bde4bf478e634931aed877ccc8db5c14
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bde4bf478e634931aed877ccc8db5c14
record_format	dspace
spelling	oai:doaj.org-article:bde4bf478e634931aed877ccc8db5c142021-11-15T15:22:21ZMixed <named-content content-type="genus-species">Chlamydia trachomatis</named-content> Peptide Antigens Provide a Specific and Sensitive Single-Well Colorimetric Enzyme-Linked Immunosorbent Assay for Detection of Human Anti<italic toggle="yes">-</italic><named-content content-type="genus-species">C. trachomatis</named-content> Antibodies10.1128/mSphere.00484-182379-5042https://doaj.org/article/bde4bf478e634931aed877ccc8db5c142018-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00484-18https://doaj.org/toc/2379-5042ABSTRACT Sensitive and specific detection of anti-Chlamydia trachomatis antibodies in standard enzyme-linked immunosorbent assays (ELISAs) is compromised by cross-reactivity and poor sensitivity of classical C. trachomatis antigens. Previously, we discovered 48 strongly reactive peptide antigens of C. trachomatis-specific B-cell epitopes from 21 immunodominant proteins. By comprehensive individual testing of 11 top-ranked peptide antigens, we found very high sensitivity and specificity for detection of anti-C. trachomatis antibodies in chemiluminescent ELISAs. The current study established a labor-saving colorimetric ELISA by using a mixture of 12 strongly reactive C. trachomatis peptide antigens (Ctr Mix1) in a single well/serum rather than assaying reactivity to each individual peptide. For performance evaluation, we used a simulated population of 212 anti-C. trachomatis antibody-positive and -negative sera from 125 women with NAAT-confirmed active C. trachomatis infection and from 87 healthy women at low risk for C. trachomatis infection. In comparison to a composite reference standard (CRS) for anti-C. trachomatis antibody status, the Ctr Mix1 IgG ELISA achieved 93.9% sensitivity, significantly superior to the 49% to 79% sensitivities of four commercial anti-C. trachomatis IgG ELISAs, and 98% specificity of all tested assays. Compared to the labor-intensive individual peptide testing, this mixed peptide ELISA retained high specificity with only marginal, ∼5% sensitivity loss. By ROC-AUC, likelihood ratio, and predictive value analyses, the Ctr Mix1 ELISA performed satisfactorily at 10% to 75% prevalence range of anti-C. trachomatis antibodies but significantly better than commercial ELISAs. Thus, the labor-saving mixed peptide colorimetric ELISA format provides simultaneously high specificity and sensitivity for detection of anti-C. trachomatis antibodies. IMPORTANCE For detection of anti-C. trachomatis antibodies by serological assays, use of classical chlamydial antigens results in high cross-reactivity and poor sensitivity. Previously, we discovered 48 strongly reactive peptide antigens of C. trachomatis-specific B-cell epitopes from 21 immunodominant proteins, and individual testing and combined scoring of 5 to 11 peptide antigens provided highly sensitive and specific detection of anti-C. trachomatis antibodies in chemiluminescent ELISAs. To simplify this method, this study established a single-well labor-saving colorimetric ELISA using a mixture of 12 strongly reactive C. trachomatis peptide antigens (Ctr Mix1) for detection of anti-C. trachomatis antibodies. This Ctr Mix1 ELISA (94% sensitivity and 98% specificity) outperformed 4 commercial ELISAs (49% to 79% sensitivity and 98% specificity). This ELISA can be easily implemented and commercialized, with convenient setup for use in nonspecialized laboratories. Thus, this mixed peptide assay with superior specificity and sensitivity will improve serodiagnosis of C. trachomatis infections.K. Shamsur RahmanToni DarvilleHarold C. WiesenfeldSharon L. HillierBernhard KaltenboeckAmerican Society for MicrobiologyarticleChlamydia trachomatisdiagnosisserologyantibody detectionB-cell epitopespeptide antigensMicrobiologyQR1-502ENmSphere, Vol 3, Iss 6 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chlamydia trachomatis diagnosis serology antibody detection B-cell epitopes peptide antigens Microbiology QR1-502
spellingShingle	Chlamydia trachomatis diagnosis serology antibody detection B-cell epitopes peptide antigens Microbiology QR1-502 K. Shamsur Rahman Toni Darville Harold C. Wiesenfeld Sharon L. Hillier Bernhard Kaltenboeck Mixed <named-content content-type="genus-species">Chlamydia trachomatis</named-content> Peptide Antigens Provide a Specific and Sensitive Single-Well Colorimetric Enzyme-Linked Immunosorbent Assay for Detection of Human Anti<italic toggle="yes">-</italic><named-content content-type="genus-species">C. trachomatis</named-content> Antibodies
description	ABSTRACT Sensitive and specific detection of anti-Chlamydia trachomatis antibodies in standard enzyme-linked immunosorbent assays (ELISAs) is compromised by cross-reactivity and poor sensitivity of classical C. trachomatis antigens. Previously, we discovered 48 strongly reactive peptide antigens of C. trachomatis-specific B-cell epitopes from 21 immunodominant proteins. By comprehensive individual testing of 11 top-ranked peptide antigens, we found very high sensitivity and specificity for detection of anti-C. trachomatis antibodies in chemiluminescent ELISAs. The current study established a labor-saving colorimetric ELISA by using a mixture of 12 strongly reactive C. trachomatis peptide antigens (Ctr Mix1) in a single well/serum rather than assaying reactivity to each individual peptide. For performance evaluation, we used a simulated population of 212 anti-C. trachomatis antibody-positive and -negative sera from 125 women with NAAT-confirmed active C. trachomatis infection and from 87 healthy women at low risk for C. trachomatis infection. In comparison to a composite reference standard (CRS) for anti-C. trachomatis antibody status, the Ctr Mix1 IgG ELISA achieved 93.9% sensitivity, significantly superior to the 49% to 79% sensitivities of four commercial anti-C. trachomatis IgG ELISAs, and 98% specificity of all tested assays. Compared to the labor-intensive individual peptide testing, this mixed peptide ELISA retained high specificity with only marginal, ∼5% sensitivity loss. By ROC-AUC, likelihood ratio, and predictive value analyses, the Ctr Mix1 ELISA performed satisfactorily at 10% to 75% prevalence range of anti-C. trachomatis antibodies but significantly better than commercial ELISAs. Thus, the labor-saving mixed peptide colorimetric ELISA format provides simultaneously high specificity and sensitivity for detection of anti-C. trachomatis antibodies. IMPORTANCE For detection of anti-C. trachomatis antibodies by serological assays, use of classical chlamydial antigens results in high cross-reactivity and poor sensitivity. Previously, we discovered 48 strongly reactive peptide antigens of C. trachomatis-specific B-cell epitopes from 21 immunodominant proteins, and individual testing and combined scoring of 5 to 11 peptide antigens provided highly sensitive and specific detection of anti-C. trachomatis antibodies in chemiluminescent ELISAs. To simplify this method, this study established a single-well labor-saving colorimetric ELISA using a mixture of 12 strongly reactive C. trachomatis peptide antigens (Ctr Mix1) for detection of anti-C. trachomatis antibodies. This Ctr Mix1 ELISA (94% sensitivity and 98% specificity) outperformed 4 commercial ELISAs (49% to 79% sensitivity and 98% specificity). This ELISA can be easily implemented and commercialized, with convenient setup for use in nonspecialized laboratories. Thus, this mixed peptide assay with superior specificity and sensitivity will improve serodiagnosis of C. trachomatis infections.
format	article
author	K. Shamsur Rahman Toni Darville Harold C. Wiesenfeld Sharon L. Hillier Bernhard Kaltenboeck
author_facet	K. Shamsur Rahman Toni Darville Harold C. Wiesenfeld Sharon L. Hillier Bernhard Kaltenboeck
author_sort	K. Shamsur Rahman
title	Mixed <named-content content-type="genus-species">Chlamydia trachomatis</named-content> Peptide Antigens Provide a Specific and Sensitive Single-Well Colorimetric Enzyme-Linked Immunosorbent Assay for Detection of Human Anti<italic toggle="yes">-</italic><named-content content-type="genus-species">C. trachomatis</named-content> Antibodies
title_short	Mixed <named-content content-type="genus-species">Chlamydia trachomatis</named-content> Peptide Antigens Provide a Specific and Sensitive Single-Well Colorimetric Enzyme-Linked Immunosorbent Assay for Detection of Human Anti<italic toggle="yes">-</italic><named-content content-type="genus-species">C. trachomatis</named-content> Antibodies
title_full	Mixed <named-content content-type="genus-species">Chlamydia trachomatis</named-content> Peptide Antigens Provide a Specific and Sensitive Single-Well Colorimetric Enzyme-Linked Immunosorbent Assay for Detection of Human Anti<italic toggle="yes">-</italic><named-content content-type="genus-species">C. trachomatis</named-content> Antibodies
title_fullStr	Mixed <named-content content-type="genus-species">Chlamydia trachomatis</named-content> Peptide Antigens Provide a Specific and Sensitive Single-Well Colorimetric Enzyme-Linked Immunosorbent Assay for Detection of Human Anti<italic toggle="yes">-</italic><named-content content-type="genus-species">C. trachomatis</named-content> Antibodies
title_full_unstemmed	Mixed <named-content content-type="genus-species">Chlamydia trachomatis</named-content> Peptide Antigens Provide a Specific and Sensitive Single-Well Colorimetric Enzyme-Linked Immunosorbent Assay for Detection of Human Anti<italic toggle="yes">-</italic><named-content content-type="genus-species">C. trachomatis</named-content> Antibodies
title_sort	mixed <named-content content-type="genus-species">chlamydia trachomatis</named-content> peptide antigens provide a specific and sensitive single-well colorimetric enzyme-linked immunosorbent assay for detection of human anti<italic toggle="yes">-</italic><named-content content-type="genus-species">c. trachomatis</named-content> antibodies
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/bde4bf478e634931aed877ccc8db5c14
work_keys_str_mv	AT kshamsurrahman mixednamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentpeptideantigensprovideaspecificandsensitivesinglewellcolorimetricenzymelinkedimmunosorbentassayfordetectionofhumanantiitalictoggleyesitalicnamedcontentcontenttypegenusspeciesctrachoma AT tonidarville mixednamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentpeptideantigensprovideaspecificandsensitivesinglewellcolorimetricenzymelinkedimmunosorbentassayfordetectionofhumanantiitalictoggleyesitalicnamedcontentcontenttypegenusspeciesctrachoma AT haroldcwiesenfeld mixednamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentpeptideantigensprovideaspecificandsensitivesinglewellcolorimetricenzymelinkedimmunosorbentassayfordetectionofhumanantiitalictoggleyesitalicnamedcontentcontenttypegenusspeciesctrachoma AT sharonlhillier mixednamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentpeptideantigensprovideaspecificandsensitivesinglewellcolorimetricenzymelinkedimmunosorbentassayfordetectionofhumanantiitalictoggleyesitalicnamedcontentcontenttypegenusspeciesctrachoma AT bernhardkaltenboeck mixednamedcontentcontenttypegenusspecieschlamydiatrachomatisnamedcontentpeptideantigensprovideaspecificandsensitivesinglewellcolorimetricenzymelinkedimmunosorbentassayfordetectionofhumanantiitalictoggleyesitalicnamedcontentcontenttypegenusspeciesctrachoma
_version_	1718428030451318784

Ejemplares similares