Restriction enzyme body doubles and PCR cloning: on the general use of type IIs restriction enzymes for cloning.
The procedure described here allows the cloning of PCR fragments containing a recognition site of the restriction endonuclease (Type IIP) used for cloning in the sequence of the insert. A Type IIS endonuclease--a Body Double of the Type IIP enzyme--is used to generate the same protruding palindrome....
Enregistré dans:
Auteurs principaux: | , , , , , , , , |
---|---|
Format: | article |
Langue: | EN |
Publié: |
Public Library of Science (PLoS)
2014
|
Sujets: | |
Accès en ligne: | https://doaj.org/article/be21df070ba745f58af2e1bafcce9e3e |
Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|