Split intein-mediated selection of cells containing two plasmids using a single antibiotic

Engineering cell lines often requires multiple plasmids with different selection markers. Here the authors present SiMPl, a method based on rationally engineered split enzymes which get reconstituted via intein-mediated protein splicing to maintain two plasmids using a single antibiotic.

Guardado en:
Detalles Bibliográficos
Autores principales: Navaneethan Palanisamy, Anna Degen, Anna Morath, Jara Ballestin Ballestin, Claudia Juraske, Mehmet Ali Öztürk, Georg A. Sprenger, Jung-Won Youn, Wolfgang W. Schamel, Barbara Di Ventura
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
Materias:
Q
Acceso en línea:https://doaj.org/article/be318edfe0da415f9f17a29b7dd12059
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!