Split intein-mediated selection of cells containing two plasmids using a single antibiotic

Engineering cell lines often requires multiple plasmids with different selection markers. Here the authors present SiMPl, a method based on rationally engineered split enzymes which get reconstituted via intein-mediated protein splicing to maintain two plasmids using a single antibiotic.

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Autores principales:	Navaneethan Palanisamy, Anna Degen, Anna Morath, Jara Ballestin Ballestin, Claudia Juraske, Mehmet Ali Öztürk, Georg A. Sprenger, Jung-Won Youn, Wolfgang W. Schamel, Barbara Di Ventura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/be318edfe0da415f9f17a29b7dd12059
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spelling	oai:doaj.org-article:be318edfe0da415f9f17a29b7dd120592021-12-02T14:38:36ZSplit intein-mediated selection of cells containing two plasmids using a single antibiotic10.1038/s41467-019-12911-12041-1723https://doaj.org/article/be318edfe0da415f9f17a29b7dd120592019-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12911-1https://doaj.org/toc/2041-1723Engineering cell lines often requires multiple plasmids with different selection markers. Here the authors present SiMPl, a method based on rationally engineered split enzymes which get reconstituted via intein-mediated protein splicing to maintain two plasmids using a single antibiotic.Navaneethan PalanisamyAnna DegenAnna MorathJara Ballestin BallestinClaudia JuraskeMehmet Ali ÖztürkGeorg A. SprengerJung-Won YounWolfgang W. SchamelBarbara Di VenturaNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-15 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Navaneethan Palanisamy Anna Degen Anna Morath Jara Ballestin Ballestin Claudia Juraske Mehmet Ali Öztürk Georg A. Sprenger Jung-Won Youn Wolfgang W. Schamel Barbara Di Ventura Split intein-mediated selection of cells containing two plasmids using a single antibiotic
description	Engineering cell lines often requires multiple plasmids with different selection markers. Here the authors present SiMPl, a method based on rationally engineered split enzymes which get reconstituted via intein-mediated protein splicing to maintain two plasmids using a single antibiotic.
format	article
author	Navaneethan Palanisamy Anna Degen Anna Morath Jara Ballestin Ballestin Claudia Juraske Mehmet Ali Öztürk Georg A. Sprenger Jung-Won Youn Wolfgang W. Schamel Barbara Di Ventura
author_facet	Navaneethan Palanisamy Anna Degen Anna Morath Jara Ballestin Ballestin Claudia Juraske Mehmet Ali Öztürk Georg A. Sprenger Jung-Won Youn Wolfgang W. Schamel Barbara Di Ventura
author_sort	Navaneethan Palanisamy
title	Split intein-mediated selection of cells containing two plasmids using a single antibiotic
title_short	Split intein-mediated selection of cells containing two plasmids using a single antibiotic
title_full	Split intein-mediated selection of cells containing two plasmids using a single antibiotic
title_fullStr	Split intein-mediated selection of cells containing two plasmids using a single antibiotic
title_full_unstemmed	Split intein-mediated selection of cells containing two plasmids using a single antibiotic
title_sort	split intein-mediated selection of cells containing two plasmids using a single antibiotic
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/be318edfe0da415f9f17a29b7dd12059
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Split intein-mediated selection of cells containing two plasmids using a single antibiotic

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