In Vitro Antioxidant Properties, Glucose-Diffusion Effects, α-Amylase Inhibitory Activity, and Antidiabetogenic Effects of C. Europaea Extracts in Experimental Animals

Caralluma europaea (Guss.) N.E.Br. (C. europaea), is a medicinal plant used traditionally to treat diabetes mellitus (DM) in Morocco. This study aimed to investigate the in vitro antioxidant properties, glucose diffusion effects, α-amylase inhibitory activity, a...

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Autores principales:	Hayat Ouassou, Mohamed Bouhrim, Noureddine Bencheikh, Mohamed Addi, Christophe Hano, Hassane Mekhfi, Abderrahim Ziyyat, Abdekhaleq Legssyer, Mohamed Aziz, Mohamed Bnouham
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Caralluma europaea</i> antidiabetogenic effect hyperglycemia glucose diffusion α-amylase antioxidant Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/be5790d0d1f64c258f7d77af1e3d2bc6
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spelling	oai:doaj.org-article:be5790d0d1f64c258f7d77af1e3d2bc62021-11-25T16:27:44ZIn Vitro Antioxidant Properties, Glucose-Diffusion Effects, α-Amylase Inhibitory Activity, and Antidiabetogenic Effects of <i>C. Europaea</i> Extracts in Experimental Animals10.3390/antiox101117472076-3921https://doaj.org/article/be5790d0d1f64c258f7d77af1e3d2bc62021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1747https://doaj.org/toc/2076-3921<i>Caralluma europaea</i> (Guss.) N.E.Br. (<i>C. europaea</i>), is a medicinal plant used traditionally to treat diabetes mellitus (DM) in Morocco. This study aimed to investigate the in vitro antioxidant properties, glucose diffusion effects, α-amylase inhibitory activity, and pancreatic protective effects of <i>C. europaea</i> in experimental alloxan-induced diabetes in mice. Total phenolic contents were determined by Folin–Ciocalteu colorimetric method, total flavonoid contents were measured by aluminum chloride colorimetric assay, and tannins contents were determined by employing the vanillin method. <i>C. europaea</i> ethyl acetate fraction exhibited high antioxidant potential in terms of radical scavenging (DPPH) (IC50 = 0.22 ± 0.01 mg/mL), β-carotene bleaching activity (IC50 = 1.153 ± 0.07 mg/mL), and Ferric-reducing antioxidant power. Glucose diffusion was significantly inhibited by the ethyl acetate fraction at 60,120and 180 min, while the aqueous extract did not have this inhibitory effect when compared with the control group. Potent α-amylase inhibitory activity was observed in the ethyl acetate fraction and the aqueous extract in vitro and in vivo using STZ-diabetic rats. On the other hand, the administration of the ethyl acetate fraction (60 mg/kg) significantly attenuated alloxan-induced death and hyperglycemia in treated mice. Furthermore, histopathological investigations revealed that the ethyl acetate fraction protected islets of Langerhans against alloxan-induced tissue alterations. These results suggest that <i>C. europaea</i> exhibited an important antihyperglycemic effect via the inhibition of glucose diffusion and pancreatic α-amylase activity. In addition, the antidiabetogenic effect of <i>C. europaea</i> might be attributed to their polyphenol and flavonoid compounds, which could be reacted alone, or in synergy, to scavenge the free radicals produced by the alloxan.Hayat OuassouMohamed BouhrimNoureddine BencheikhMohamed AddiChristophe HanoHassane MekhfiAbderrahim ZiyyatAbdekhaleq LegssyerMohamed AzizMohamed BnouhamMDPI AGarticle<i>Caralluma europaea</i>antidiabetogenic effecthyperglycemiaglucose diffusionα-amylaseantioxidantTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1747, p 1747 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Caralluma europaea</i> antidiabetogenic effect hyperglycemia glucose diffusion α-amylase antioxidant Therapeutics. Pharmacology RM1-950
spellingShingle	<i>Caralluma europaea</i> antidiabetogenic effect hyperglycemia glucose diffusion α-amylase antioxidant Therapeutics. Pharmacology RM1-950 Hayat Ouassou Mohamed Bouhrim Noureddine Bencheikh Mohamed Addi Christophe Hano Hassane Mekhfi Abderrahim Ziyyat Abdekhaleq Legssyer Mohamed Aziz Mohamed Bnouham In Vitro Antioxidant Properties, Glucose-Diffusion Effects, α-Amylase Inhibitory Activity, and Antidiabetogenic Effects of <i>C. Europaea</i> Extracts in Experimental Animals
description	<i>Caralluma europaea</i> (Guss.) N.E.Br. (<i>C. europaea</i>), is a medicinal plant used traditionally to treat diabetes mellitus (DM) in Morocco. This study aimed to investigate the in vitro antioxidant properties, glucose diffusion effects, α-amylase inhibitory activity, and pancreatic protective effects of <i>C. europaea</i> in experimental alloxan-induced diabetes in mice. Total phenolic contents were determined by Folin–Ciocalteu colorimetric method, total flavonoid contents were measured by aluminum chloride colorimetric assay, and tannins contents were determined by employing the vanillin method. <i>C. europaea</i> ethyl acetate fraction exhibited high antioxidant potential in terms of radical scavenging (DPPH) (IC50 = 0.22 ± 0.01 mg/mL), β-carotene bleaching activity (IC50 = 1.153 ± 0.07 mg/mL), and Ferric-reducing antioxidant power. Glucose diffusion was significantly inhibited by the ethyl acetate fraction at 60,120and 180 min, while the aqueous extract did not have this inhibitory effect when compared with the control group. Potent α-amylase inhibitory activity was observed in the ethyl acetate fraction and the aqueous extract in vitro and in vivo using STZ-diabetic rats. On the other hand, the administration of the ethyl acetate fraction (60 mg/kg) significantly attenuated alloxan-induced death and hyperglycemia in treated mice. Furthermore, histopathological investigations revealed that the ethyl acetate fraction protected islets of Langerhans against alloxan-induced tissue alterations. These results suggest that <i>C. europaea</i> exhibited an important antihyperglycemic effect via the inhibition of glucose diffusion and pancreatic α-amylase activity. In addition, the antidiabetogenic effect of <i>C. europaea</i> might be attributed to their polyphenol and flavonoid compounds, which could be reacted alone, or in synergy, to scavenge the free radicals produced by the alloxan.
format	article
author	Hayat Ouassou Mohamed Bouhrim Noureddine Bencheikh Mohamed Addi Christophe Hano Hassane Mekhfi Abderrahim Ziyyat Abdekhaleq Legssyer Mohamed Aziz Mohamed Bnouham
author_facet	Hayat Ouassou Mohamed Bouhrim Noureddine Bencheikh Mohamed Addi Christophe Hano Hassane Mekhfi Abderrahim Ziyyat Abdekhaleq Legssyer Mohamed Aziz Mohamed Bnouham
author_sort	Hayat Ouassou
title	In Vitro Antioxidant Properties, Glucose-Diffusion Effects, α-Amylase Inhibitory Activity, and Antidiabetogenic Effects of <i>C. Europaea</i> Extracts in Experimental Animals
title_short	In Vitro Antioxidant Properties, Glucose-Diffusion Effects, α-Amylase Inhibitory Activity, and Antidiabetogenic Effects of <i>C. Europaea</i> Extracts in Experimental Animals
title_full	In Vitro Antioxidant Properties, Glucose-Diffusion Effects, α-Amylase Inhibitory Activity, and Antidiabetogenic Effects of <i>C. Europaea</i> Extracts in Experimental Animals
title_fullStr	In Vitro Antioxidant Properties, Glucose-Diffusion Effects, α-Amylase Inhibitory Activity, and Antidiabetogenic Effects of <i>C. Europaea</i> Extracts in Experimental Animals
title_full_unstemmed	In Vitro Antioxidant Properties, Glucose-Diffusion Effects, α-Amylase Inhibitory Activity, and Antidiabetogenic Effects of <i>C. Europaea</i> Extracts in Experimental Animals
title_sort	in vitro antioxidant properties, glucose-diffusion effects, α-amylase inhibitory activity, and antidiabetogenic effects of <i>c. europaea</i> extracts in experimental animals
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/be5790d0d1f64c258f7d77af1e3d2bc6
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In Vitro Antioxidant Properties, Glucose-Diffusion Effects, α-Amylase Inhibitory Activity, and Antidiabetogenic Effects of <i>C. Europaea</i> Extracts in Experimental Animals

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