Clonality despite sex: the evolution of host-associated sexual neighborhoods in the pathogenic fungus Penicillium marneffei.
Molecular genetic approaches typically detect recombination in microbes regardless of assumed asexuality. However, genetic data have shown the AIDS-associated pathogen Penicillium marneffei to have extensive spatial genetic structure at local and regional scales, and although there has been some gen...
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2012
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oai:doaj.org-article:bf0903e2533c4b0db3b7da4c231e15092021-11-18T06:06:31ZClonality despite sex: the evolution of host-associated sexual neighborhoods in the pathogenic fungus Penicillium marneffei.1553-73661553-737410.1371/journal.ppat.1002851https://doaj.org/article/bf0903e2533c4b0db3b7da4c231e15092012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23055919/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Molecular genetic approaches typically detect recombination in microbes regardless of assumed asexuality. However, genetic data have shown the AIDS-associated pathogen Penicillium marneffei to have extensive spatial genetic structure at local and regional scales, and although there has been some genetic evidence that a sexual cycle is possible, this haploid fungus is thought to be genetically, as well as morphologically, asexual in nature because of its highly clonal population structure. Here we use comparative genomics, experimental mixed-genotype infections, and population genetic data to elucidate the role of recombination in natural populations of P. marneffei. Genome wide comparisons reveal that all the genes required for meiosis are present in P. marneffei, mating type genes are arranged in a similar manner to that found in other heterothallic fungi, and there is evidence of a putatively meiosis-specific mutational process. Experiments suggest that recombination between isolates of compatible mating types may occur during mammal infection. Population genetic data from 34 isolates from bamboo rats in India, Thailand and Vietnam, and 273 isolates from humans in China, India, Thailand, and Vietnam show that recombination is most likely to occur across spatially and genetically limited distances in natural populations resulting in highly clonal population structure yet sexually reproducing populations. Predicted distributions of three different spatial genetic clusters within P. marneffei overlap with three different bamboo rat host distributions suggesting that recombination within hosts may act to maintain population barriers within P. marneffei.Daniel A HenkRevital Shahar-GolanKhuraijam Ranjana DeviKylie J BoyceNengyong ZhanNatalie D FedorovaWilliam C NiermanPo-Ren HsuehKwok-Yung YuenTran P M SieuNguyen Van KinhHeiman WertheimStephen G BakerJeremy N DayNongnuch VanittanakomElaine M BignellAlex AndrianopoulosMatthew C FisherPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 8, Iss 10, p e1002851 (2012) |
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| topic |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 |
| spellingShingle |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Daniel A Henk Revital Shahar-Golan Khuraijam Ranjana Devi Kylie J Boyce Nengyong Zhan Natalie D Fedorova William C Nierman Po-Ren Hsueh Kwok-Yung Yuen Tran P M Sieu Nguyen Van Kinh Heiman Wertheim Stephen G Baker Jeremy N Day Nongnuch Vanittanakom Elaine M Bignell Alex Andrianopoulos Matthew C Fisher Clonality despite sex: the evolution of host-associated sexual neighborhoods in the pathogenic fungus Penicillium marneffei. |
| description |
Molecular genetic approaches typically detect recombination in microbes regardless of assumed asexuality. However, genetic data have shown the AIDS-associated pathogen Penicillium marneffei to have extensive spatial genetic structure at local and regional scales, and although there has been some genetic evidence that a sexual cycle is possible, this haploid fungus is thought to be genetically, as well as morphologically, asexual in nature because of its highly clonal population structure. Here we use comparative genomics, experimental mixed-genotype infections, and population genetic data to elucidate the role of recombination in natural populations of P. marneffei. Genome wide comparisons reveal that all the genes required for meiosis are present in P. marneffei, mating type genes are arranged in a similar manner to that found in other heterothallic fungi, and there is evidence of a putatively meiosis-specific mutational process. Experiments suggest that recombination between isolates of compatible mating types may occur during mammal infection. Population genetic data from 34 isolates from bamboo rats in India, Thailand and Vietnam, and 273 isolates from humans in China, India, Thailand, and Vietnam show that recombination is most likely to occur across spatially and genetically limited distances in natural populations resulting in highly clonal population structure yet sexually reproducing populations. Predicted distributions of three different spatial genetic clusters within P. marneffei overlap with three different bamboo rat host distributions suggesting that recombination within hosts may act to maintain population barriers within P. marneffei. |
| format |
article |
| author |
Daniel A Henk Revital Shahar-Golan Khuraijam Ranjana Devi Kylie J Boyce Nengyong Zhan Natalie D Fedorova William C Nierman Po-Ren Hsueh Kwok-Yung Yuen Tran P M Sieu Nguyen Van Kinh Heiman Wertheim Stephen G Baker Jeremy N Day Nongnuch Vanittanakom Elaine M Bignell Alex Andrianopoulos Matthew C Fisher |
| author_facet |
Daniel A Henk Revital Shahar-Golan Khuraijam Ranjana Devi Kylie J Boyce Nengyong Zhan Natalie D Fedorova William C Nierman Po-Ren Hsueh Kwok-Yung Yuen Tran P M Sieu Nguyen Van Kinh Heiman Wertheim Stephen G Baker Jeremy N Day Nongnuch Vanittanakom Elaine M Bignell Alex Andrianopoulos Matthew C Fisher |
| author_sort |
Daniel A Henk |
| title |
Clonality despite sex: the evolution of host-associated sexual neighborhoods in the pathogenic fungus Penicillium marneffei. |
| title_short |
Clonality despite sex: the evolution of host-associated sexual neighborhoods in the pathogenic fungus Penicillium marneffei. |
| title_full |
Clonality despite sex: the evolution of host-associated sexual neighborhoods in the pathogenic fungus Penicillium marneffei. |
| title_fullStr |
Clonality despite sex: the evolution of host-associated sexual neighborhoods in the pathogenic fungus Penicillium marneffei. |
| title_full_unstemmed |
Clonality despite sex: the evolution of host-associated sexual neighborhoods in the pathogenic fungus Penicillium marneffei. |
| title_sort |
clonality despite sex: the evolution of host-associated sexual neighborhoods in the pathogenic fungus penicillium marneffei. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/bf0903e2533c4b0db3b7da4c231e1509 |
| work_keys_str_mv |
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