Ex vivo visualization of RNA polymerase III-specific gene activity with electron microscopy

Manger, Ermel, and Frangakis report the use of CRISPR/Cas9 to stably insert multiple copies of a particular gene-of-interest near the 35S rRNA gene, to allow direct visualization of gene clusters with electron microscopy. They achieve more than 7-fold enrichment of associated Pol III complexes withi...

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Autores principales:	Sina Manger, Utz H. Ermel, Achilleas S. Frangakis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bf196ee91b9141eaaa696f911e1f0986
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spelling	oai:doaj.org-article:bf196ee91b9141eaaa696f911e1f09862021-12-02T14:21:58ZEx vivo visualization of RNA polymerase III-specific gene activity with electron microscopy10.1038/s42003-021-01752-82399-3642https://doaj.org/article/bf196ee91b9141eaaa696f911e1f09862021-02-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01752-8https://doaj.org/toc/2399-3642Manger, Ermel, and Frangakis report the use of CRISPR/Cas9 to stably insert multiple copies of a particular gene-of-interest near the 35S rRNA gene, to allow direct visualization of gene clusters with electron microscopy. They achieve more than 7-fold enrichment of associated Pol III complexes within the field of view, demonstrating the utility of the approach.Sina MangerUtz H. ErmelAchilleas S. FrangakisNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Sina Manger Utz H. Ermel Achilleas S. Frangakis Ex vivo visualization of RNA polymerase III-specific gene activity with electron microscopy
description	Manger, Ermel, and Frangakis report the use of CRISPR/Cas9 to stably insert multiple copies of a particular gene-of-interest near the 35S rRNA gene, to allow direct visualization of gene clusters with electron microscopy. They achieve more than 7-fold enrichment of associated Pol III complexes within the field of view, demonstrating the utility of the approach.
format	article
author	Sina Manger Utz H. Ermel Achilleas S. Frangakis
author_facet	Sina Manger Utz H. Ermel Achilleas S. Frangakis
author_sort	Sina Manger
title	Ex vivo visualization of RNA polymerase III-specific gene activity with electron microscopy
title_short	Ex vivo visualization of RNA polymerase III-specific gene activity with electron microscopy
title_full	Ex vivo visualization of RNA polymerase III-specific gene activity with electron microscopy
title_fullStr	Ex vivo visualization of RNA polymerase III-specific gene activity with electron microscopy
title_full_unstemmed	Ex vivo visualization of RNA polymerase III-specific gene activity with electron microscopy
title_sort	ex vivo visualization of rna polymerase iii-specific gene activity with electron microscopy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bf196ee91b9141eaaa696f911e1f0986
work_keys_str_mv	AT sinamanger exvivovisualizationofrnapolymeraseiiispecificgeneactivitywithelectronmicroscopy AT utzhermel exvivovisualizationofrnapolymeraseiiispecificgeneactivitywithelectronmicroscopy AT achilleassfrangakis exvivovisualizationofrnapolymeraseiiispecificgeneactivitywithelectronmicroscopy
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Ex vivo visualization of RNA polymerase III-specific gene activity with electron microscopy

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