Optimised metrics for CRISPR-KO screens with second-generation gRNA libraries
Abstract Genome-wide CRISPR-based knockout (CRISPR-KO) screening is an emerging technique which enables systematic genetic analysis of a cellular or molecular phenotype in question. Continuous improvements, such as modifications to the guide RNA (gRNA) scaffold and the development of gRNA on-target...
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Autores principales: | Swee Hoe Ong, Yilong Li, Hiroko Koike-Yusa, Kosuke Yusa |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2017
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Materias: | |
Acceso en línea: | https://doaj.org/article/bf61f265b7c34397b4f245df7adcb64e |
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